Laser-Induced Mouse Model of Chronic Ocular Hypertension

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Grozdanic, Sinisa
Betts, Daniel
Kwon, Young
Kardon, Randy
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Sakaguchi, Donald
Director of Biology and Genetics Undergraduate Program and Morrill Professor
Allbaugh, Rachel
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Biomedical Sciences

The Department of Biomedical Sciences aims to provide knowledge of anatomy and physiology in order to understand the mechanisms and treatment of animal diseases. Additionally, it seeks to teach the understanding of drug-action for rational drug-therapy, as well as toxicology, pharmacodynamics, and clinical drug administration.

The Department of Biomedical Sciences was formed in 1999 as a merger of the Department of Veterinary Anatomy and the Department of Veterinary Physiology and Pharmacology.

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  • College of Veterinary Medicine (parent college)
  • Department of Veterinary Anatomy (predecessor, 1997)
  • Department of Veterinary Physiology and Pharmacology (predecessor, 1997)

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Veterinary Clinical Sciences
The mission of the Veterinary Clinical Sciences Department and the Veterinary Medical Center is to be strong academically, to provide outstanding services, and to conduct research in the multiple areas of Veterinary Clinical Sciences. Our goals are to teach students in the multiple disciplines of Veterinary Clinical Sciences, to provide excellent veterinary services to clients, and to generate and disseminate new knowledge in the areas of Veterinary Clinical Sciences. Our objectives are to provide a curriculum in the various aspects of Veterinary Clinical Sciences which ensures students acquire the skills and knowledge to be successful in their chosen careers. We also strive to maintain a caseload of sufficient size and diversity which insures a broad clinical experience for students, residents, and faculty. In addition, we aim to provide clinical veterinary services of the highest standards to animal owners and to referring veterinarians. And finally, we strive to provide an environment and opportunities which foster and encourage the generation and dissemination of new knowledge in many of the disciplines of Veterinary Clinical Sciences.
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purpose. To develop an inducible mouse model of glaucoma.

methods. An obstruction of aqueous humor outflow in adult C57BL6/J mice was induced by combined injection of indocyanine green (ICG) dye into the anterior chamber and diode laser treatment. To evaluate intraocular pressure (IOP), tonometry was performed with a modified Goldmann tonometer. The function of the retina was evaluated with electroretinography (ERG).

results. IOP was significantly elevated in surgical eyes compared with control eyes: before surgery, 15.2 ± 0.6 mm Hg; 10 days after surgery, 33.6 ± 1.5 mm Hg (P < 0.001); and 30 days after surgery, 27.4 ± 1.2 mm Hg (P < 0.001). However, 60 days after surgery, IOP in the surgical eyes decreased to 19.5 ± 0.9 mm Hg and was not significantly different compared with control eyes (control, 17.3 ± 0.7 mm Hg; P = 0.053). ERG amplitudes, expressed as a ratio (surgical/control), were decreased in surgical eyes. The amplitudes for b-wave were: before surgery, 107.6% ± 4.6%; 28 days after surgery, 61% ± 4% (P < 0.001); and 56 days after surgery, 62% ± 5.6% (P < 0.001). Oscillatory potentials were the most dramatically affected: before surgery, 108.6% ± 6.7%; 28 days after surgery, 57.5% ± 5% (P < 0.01); and 56 days after surgery, 57% ± 8.5% (P < 0.001). Amplitudes of the a-waves had relatively smaller but still significant deficits: before surgery, 105.8% ± 6.9%; 28 days after surgery, 72.2% ± 5.4% (P < 0.01); and 56 days after surgery, 79.8% ± 11.0% (P < 0.01). Histologic analysis of the surgical eyes revealed development of anterior synechia, loss of retinal ganglion cells (RGCs), and thinning of all retinal layers. Electron microscopy of optic nerve cross sections revealed swelling and degeneration of the large diameter axons and gliosis.

conclusions. Diode laser treatment of ICG saturated episcleral veins causes a chronic elevation of IOP and sustained ERG deficits.


This article is from Investigative Ophthalmology & Visual Science 44 (2003): 4337, doi: 10.1167/iovs.03-0015. Posted with permission.

Wed Jan 01 00:00:00 UTC 2003