Identification and characterization of a putative telomere end-binding protein from Tetrahymena thermophila Dobbs, Drena Hou, Zhen Schierer, Ted Henderson, Eric Henderson, Eric
dc.contributor.department Zoology and Genetics 2018-02-18T05:05:41.000 2020-07-07T05:16:46Z 2020-07-07T05:16:46Z Sun Jan 01 00:00:00 UTC 1995 1995-03-01
dc.description.abstract <p>Telomeric DNA of Tetrahymena thermophila consists of a long stretch of (TTGGGG)n double-stranded repeats with a single-stranded (TTGGGG)2 3' overhang at the end of the chromosome. We have identified and characterized a protein that specifically binds to a synthetic telomeric substrate consisting of duplex DNA and the 3' telomeric repeat overhang. This protein is called TEP (telomere end-binding protein). A change from G to A in the third position of the TTGGGG overhang repeat converts the substrate to a human telomere analog and reduces the binding affinity approximately threefold. Changing two G's to C's in the TTGGGG repeats totally abolishes binding. However, permutation of the Tetrahymena repeat sequence has only a minor effect on binding. A duplex structure adjacent to the 3' overhang is required for binding, although the duplex need not contain telomeric repeats. TEP does not bind to G-quartet DNA, which is formed by many G-rich sequences. TEP has a greatly reduced affinity for RNA substrates. The copy number of TEP is at least 2 x 10(4) per cell, and it is present under different conditions of cell growth and development, although its level varies. UV cross-linking experiments show that TEP has an apparent molecular mass of approximately 65 kDa. Unlike other telomere end-binding proteins, TEP is sensitive to high salt concentrations.</p>
dc.description.comments <p>This article is from <em>Molecular and Cellular Biology </em>15 (1995): 1144, doi: <a href="" target="_blank">10.1128/MCB.15.3.1144</a>. Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1010
dc.identifier.contextkey 9761215
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dc.identifier.submissionpath zool_pubs/14
dc.language.iso en
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dc.source.uri 10.1128/MCB.15.3.1144
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Genetics
dc.subject.disciplines Genomics
dc.subject.disciplines Zoology
dc.title Identification and characterization of a putative telomere end-binding protein from Tetrahymena thermophila
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication 68bc511a-afd6-4353-ae20-066de33682b9
relation.isOrgUnitOfPublication 4a2929da-5374-4338-b62f-f5fd9e156ef9
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