The Maize Unstable factor for orange1 Is a Dominant Epigenetic Modifier of a Tissue Specifically Silent Allele of pericarp color1

dc.contributor.author Chopra, Surinder
dc.contributor.author Cocciolone, Suzy
dc.contributor.author Bushman, Shaun
dc.contributor.author Sangar, Vineet
dc.contributor.author McMullen, Michael
dc.contributor.author Peterson, Thomas
dc.contributor.department Zoology
dc.contributor.department Agronomy
dc.date 2018-02-18T10:48:45.000
dc.date.accessioned 2020-07-07T05:16:56Z
dc.date.available 2020-07-07T05:16:56Z
dc.date.issued 2003-03-01
dc.description.abstract <p>We have characterized <em>Unstable factor for orange1</em> (<em>Ufo1</em>), a dominant, allele-specific modifier of expression of the maize <em>pericarp color1</em> (<em>p1</em>) gene. The <em>p1</em> gene encodes an <em>Myb</em>-homologous transcriptional activator of genes required for biosynthesis of red phlobaphene pigments. The <em>P1-wr</em> allele specifies colorless kernel pericarp and red cobs, whereas <em>Ufo1</em> modifies <em>P1-wr</em> expression to confer pigmentation in kernel pericarp, as well as vegetative tissues, which normally do not accumulate significant amounts of phlobaphene pigments. In the presence of <em>Ufo1, P1-wr</em> transcript levels and transcription rate are increased in kernel pericarp. The <em>P1-wr</em> allele contains approximately six <em>p1</em> gene copies present in a hypermethylated and multicopy tandem array. In <em>P1-wr Ufo1</em> plants, methylation of <em>P1-wr</em> DNA sequences is reduced, whereas the methylation state of other repetitive genomic sequences was not detectably affected. The phenotypes produced by the interaction of <em>P1-wr</em> and <em>Ufo1</em> are unstable, exhibiting somatic mosaicism and variable penetrance. Moreover, the changes in <em>P1-wr</em> expression and methylation are not heritable: meiotic segregants that lack <em>Ufo1</em> revert to the normal <em>P1-wr</em> expression and methylation patterns. These results demonstrate the existence of a class of modifiers of gene expression whose effects are associated with transient changes in DNA methylation of specific loci.</p>
dc.description.comments <p>This article is from <em>Genetics </em>163 (2003): 1135.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/zool_pubs/40/
dc.identifier.articleid 1040
dc.identifier.contextkey 10168773
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath zool_pubs/40
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92651
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/40/2003_Peterson_MaizeUnstable.pdf|||Sat Jan 15 00:07:24 UTC 2022
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.title The Maize Unstable factor for orange1 Is a Dominant Epigenetic Modifier of a Tissue Specifically Silent Allele of pericarp color1
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication ce966761-2eda-46df-bdb8-30787bedf687
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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