The Characterization of Salmonella isolated from Pig Meat in Northern Ireland by PFGE and Antibiotic Resistance Profiles

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2007-01-01
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Egan, D.
Spense, S.
Dooley, J.
Naughton, P.
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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The emergence of antibiotic resistance and especially multiple antibiotic resistance Salmonella has become a concern for the pig industry throughout the EU. Pig herds and pork are considered as principal reservoirs for the multi-resistant Salmonella type Typhimunum DT104, which has acquired resistance to ampicillin, chloramphenicol, streptomycm, sulphonamides and tetracycline. This resistance pattern is also known as ACSSuT. In this study Porcine Salmonella strains were isolated between December 2005 and December 2006. The strains origmated from an abattoir study sampling the 'oyster' cut. The Antimicrobial Resistance Profiles of all Salmonella isolates in this study were determined by disk diffusion tests. Twelve antibiotics were utilized throughout the profiling procedure Pulsed Field Gel Electrophoresis (PFGE), which is regarded as the "Gold Standard" for the typmg and strain identification of Salmonella isolates, was used to determine DNA fingerprints of the Salmonella isolates using the restriction enzyme Xbal (Invitrogen) The fragments were then separated by PFGE in a Chef DR II system (Bio-Rad). This enabled comparison of Salmonella isolated in this study.

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Mon Jan 01 00:00:00 UTC 2007