Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing

dc.contributor.author Palić, Dušan
dc.contributor.author Andreasen, Claire
dc.contributor.author Beck, Linda
dc.contributor.author Palić, Jelena
dc.contributor.author Andreasen, Claire
dc.contributor.department Veterinary Pathology
dc.date 2018-02-16T19:39:29.000
dc.date.accessioned 2020-07-07T05:16:05Z
dc.date.available 2020-07-07T05:16:05Z
dc.date.issued 2011-02-01
dc.description.abstract <p>Studies of innate immunity in fish species of special concern are essential for better understanding of their health status during hatchery rearing conditions. The cytochemical and morphological characterizations of blood granulocytes have been used to provide information about phylogenetic differences and determine the potential use of neutrophil function assays. Rapid, simple, cytochemical staining kits used routinely for staining mammalian granulocytes have been used to characterize granulocytes from blood of four fish species: Arctic grayling, cutthroat trout, June sucker, and shovelnose sturgeon. Blood smears were stained with Peroxidase 391 (myeloperoxidase, MPO), alkaline phosphatase (AP), Periodic Acid Schiff (PAS) and Diff-quick stain; examined using bright field and differential interference contrast microscopy. Granulocytes on blood smears were evaluated based on the cell morphology, and presence or absence of the specific chromogen. Presence of lymphocytes, monocytes, platelets/thrombocytes and granulocytes was determined in all fish species. Arctic grayling, June sucker, and cutthroat trout had MPO positive granulocytes, while shovelnose sturgeon heterophils had positive reaction for leukocyte AP, but not MPO. Presence of MPO indicated potential to measure oxidative burst and degranulation of neutrophil primary granules in Arctic grayling, cutthroat trout and June sucker. Absence of MPO in shovelnose sturgeon suggested use of different enzyme marker (AP) in degranulation assay for this species. Standardization of cytochemical techniques allowed forrapid screening of leukocyte types, reducing the number of fish, time and effort to select adequate neutrophil function assays to be used in studies of health status in species of special concern.</p>
dc.description.comments <p>This article is from <em>Fish & Shellfish Immunology</em> 30 (2011: 646, doi:<a href="http://dx.doi.org/10.1016/j.fsi.2010.12.024" target="_blank">10.1016/j.fsi.2010.12.024</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/71/
dc.identifier.articleid 1074
dc.identifier.contextkey 7358216
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/71
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92499
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/71/2011_Andreasen_UseRapidCytochemical.pdf|||Sat Jan 15 01:42:07 UTC 2022
dc.source.uri 10.1016/j.fsi.2010.12.024
dc.subject.disciplines Aquaculture and Fisheries
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Fish species of special concern
dc.subject.keywords leukocyte morphology
dc.subject.keywords cytochemical characterization
dc.subject.keywords granulocyte function
dc.title Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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