Integrating metabolomics and transcriptomics data to discover a biocatalyst that can generate the amine precursors for alkamide biosynthesis

Date
2016-12-01
Authors
Nikolau, Basil
Rizhsky, Ludmila
Jin, Huanan
Shepard, Michael
Scott, Harry
Teitgen, Alicen
Wurtele, Eve
Perera, M. Ann
Mhaske, Vandana
Jose, Adarsh
Zheng, Xiaobin
Crispin, Matt
Wurtele, Eve
Jones, Dallas
Hur, Manhoi
Góngora-Castillo, Elsa
Buell, C. Robin
Minto, Robert
Nikolau, Basil
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Biochemistry, Biophysics and Molecular BiologyOffice of BiotechnologyGenetics, Development and Cell BiologyOffice of Biotechnology
Abstract

The Echinacea genus is exemplary of over 30 plant families that produce a set of bioactive amides, called alkamides. The Echinacea alkamides may be assembled from two distinct moieties, a branched-chain amine that is acylated with a novel polyunsaturated fatty acid. In this study we identified the potential enzymological source of the amine moiety as a pyridoxal phosphate dependent decarboxylating enzyme that uses branched chain amino acids as substrate. This identification was based on a correlative analysis of the transcriptomes and metabolomes of 36 different E. purpurea tissues and organs, which expressed distinct alkamide profiles. Although no correlation was found between the accumulation patterns of the alkamides and their putative metabolic precursors (i.e., fatty acids and branched chain amino acids), isotope-labeling analyses supported the transformation of valine and isoleucine to isobutylamine and 2-methylbutylamine as reactions of alkamide biosynthesis. Sequence homology identified the pyridoxal phosphate dependent decarboxylase-like proteins in the translated proteome of E. purpurea. These sequences were prioritized for direct characterization by correlating their transcript levels with alkamide accumulation patterns in different organs and tissues, and this multi-pronged approach led to the identification and characterization of a branched-chain amino acid decarboxylase, which would appear to be responsible for generating the amine moieties of naturally occurring alkamides.

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This is a manuscript of an article published as Rizhsky, Ludmila, Huanan Jin, Michael R. Shepard, Harry W. Scott, Alicen M. Teitgen, M. Ann Perera, Vandana Mhaske et al. "Integrating metabolomics and transcriptomics data to discover a biocatalyst that can generate the amine precursors for alkamide biosynthesis." The Plant Journal 88, no. 5 (2016): 775-793. doi: 10.1111/tpj.13295. Posted with permission.

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