High spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of maize leaf

dc.contributor.author Nikolau, Basil
dc.contributor.author Yandeau-Nelson, Marna
dc.contributor.author Duenas, Maria Emilia
dc.contributor.author Klein, Adam
dc.contributor.author Alexander, Liza
dc.contributor.author Lee, Young Jin
dc.contributor.author Yandeau-Nelson, Marna
dc.contributor.author Nikolau, Basil
dc.contributor.author Lee, Young Jin
dc.contributor.department Ames Laboratory
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemistry
dc.contributor.department Ames Laboratory
dc.contributor.department Center for Metabolic Biology
dc.date 2018-05-30T22:40:37.000
dc.date.accessioned 2020-06-30T04:01:47Z
dc.date.available 2020-06-30T04:01:47Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2017-02-01
dc.description.abstract <p>Metabolism in plants is compartmentalized among different tissues, cells and subcellular organelles. Mass spectrometry imaging (MSI) with matrix-assisted laser desorption ionization (MALDI) has recently advanced to allow for the visualization of metabolites at single cell resolution. Here we applied 5 and 10 m high-spatial resolution MALDI-MSI to the asymmetric Kranz anatomy of maize leaves to study the differential localization of two major anionic lipids in thylakoid membranes, sulfoquinovosyldiacylglycerols (SQDG) and phosphatidylglycerols (PG). The quantification and localization of SQDG and PG molecular species, among mesophyll (M) and bundle sheath (BS) cells, are compared across the leaf developmental gradient from four maize genotypes (the inbreds B73 and Mo17, and reciprocal hybrids B73xMo17 and Mo17xB73). SQDG species are uniformly distributed in both photosynthetic cell types regardless of leaf development or genotype. However, PG shows photosynthetic cell-specific differential localization depending on the genotype and the fatty acyl chain constituent. Overall, 16:1-containing PGs primarily contribute to the thylakoid membranes of M cells while BS chloroplasts are mostly composed of 16:0-containing PGs. Furthermore, PG 32:0 shows genotype-specific differences in cellular distribution, with preferential localization in BS cells for B73, but more uniform distribution between BS and M cells in Mo17. Maternal inheritance is exhibited within the hybrids such that localization of PG 32:0 in B73xMo17 is similar to the distribution in the B73 parental inbred, whereas that of Mo17xB73 resembles the Mo17 parent. This study demonstrates the power of MALDI-MSI to reveal unprecedented insights on metabolic outcomes in multicellular organisms at single cell resolution.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Dueñas, Maria Emilia, Adam T. Klein, Liza E. Alexander, Marna D. Yandeau‐Nelson, Basil J. Nikolau, and Young Jin Lee. "High spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of maize leaf." <em>The Plant Journal</em> 89, no. 4 (2017): 825-838, which has been published in final form at doi: <a href="https://doi.org/10.1111/tpj.13422">10.1111/tpj.13422</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/197/
dc.identifier.articleid 1201
dc.identifier.contextkey 12220074
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/197
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37869
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/197/2017_Nelson_HighSpatialManuscript.pdf|||Fri Jan 14 21:59:13 UTC 2022
dc.source.uri 10.1111/tpj.13422
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords mass spectrometry imaging
dc.subject.keywords Kranz anatomy
dc.subject.keywords C4 plants
dc.subject.keywords Zea mays L.
dc.subject.keywords bundle sheath
dc.subject.keywords mesophyll
dc.subject.keywords B73
dc.subject.keywords Mo17
dc.subject.keywords membrane lipids
dc.subject.keywords single cell
dc.title High spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of maize leaf
dc.type article
dc.type.genre article
dspace.entity.type Publication
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