Variation in seed dormancy in Echinochloa and the development of a standard protocol for germination testing. II: Breaking dormancy in seeds unresponsive to light or dark conditions alone by using heat and ethanol pretreatment

dc.contributor.author Widrlechner, Mark
dc.contributor.author Kovach, David
dc.contributor.author Widrlechner, Mark
dc.contributor.author Brenner, David
dc.contributor.author Brenner, David
dc.contributor.department North Central Regional Plant Introduction Station
dc.date 2018-02-13T06:02:04.000
dc.date.accessioned 2020-06-30T06:10:39Z
dc.date.available 2020-06-30T06:10:39Z
dc.date.embargo 2013-02-09
dc.date.issued 2012-10-01
dc.description.abstract <p>A recently established method for the germination of <em>Echinochloa</em> seeds recognised and accounted for variation in responses to light and darkness. This method used parallel light and dark tests and was successful in promoting germination in most seed lots. However, some samples exhibited deeper dormancy and were not fully responsive to either test. In the present study, we employed warm pretreatments where seeds were exposed to dilute aqueous ethanol solutions to attempt to break their dormancy. Based on tests of five <em>Echinochloa</em> accessions, we propose a new, follow-up protocol that can be used on samples unresponsive to the established method. The additional step involves a 3-day dark pretreatment at 35-37°C, imbibing the seeds in ∼0.25M ethanol (aq). After this pretreatment, the seeds should be germinated for 14 days at 20/30°C (16 hours/ 8 hours) with half held in darkness and the other half exposed to an 8-hour light cycle.</p>
dc.description.comments <p>This article is from S<em>eed Science and Technology </em>40 (2012): 299–308.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ncrpis_pubs/19/
dc.identifier.articleid 1022
dc.identifier.contextkey 3674113
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ncrpis_pubs/19
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/55996
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ncrpis_pubs/19/Widrlechner_2012_VariationSeedDormancy.pdf|||Fri Jan 14 21:48:36 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Ecology and Evolutionary Biology
dc.subject.disciplines Horticulture
dc.subject.disciplines Plant Sciences
dc.subject.keywords Agronomy
dc.subject.keywords Ecology Evolution and Organismal Biology
dc.subject.keywords Horticulture
dc.title Variation in seed dormancy in Echinochloa and the development of a standard protocol for germination testing. II: Breaking dormancy in seeds unresponsive to light or dark conditions alone by using heat and ethanol pretreatment
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication a40d8542-37dd-4fef-9a9e-521123110cc9
relation.isOrgUnitOfPublication 8dcf3b33-db2f-46f8-8b80-20844d33fa84
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