Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode

dc.contributor.author Hermsmeier, Dieter
dc.contributor.author Baum, Thomas
dc.contributor.author Mazarei, Mitra
dc.contributor.author Baum, Thomas
dc.contributor.department Plant Pathology and Microbiology
dc.date 2018-02-18T18:48:53.000
dc.date.accessioned 2020-06-30T06:22:27Z
dc.date.available 2020-06-30T06:22:27Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 1998
dc.date.issued 1998-12-01
dc.description.abstract <p>The marked cellular changes during feeding site formation of the soybean cyst nematode (<em>Heterodera glycines</em>) indicate major changes in soybean gene expression. We used differential display of mRNA to detect host gene expression changes during the early compatible interaction between soybean and <em>H. glycines</em>. Fifteen cDNA clones corresponding to mRNAs with different abundances in <em>H. glycines</em>-infected versus uninfected roots were identified. Differential display results indicated that abundances of five mRNAs increased in infected roots, whereas abundances of 10 mRNAs decreased. Transcripts for nine of these 15 cDNAs could be detected on RNA blots, and their hybridization signals confirmed the differential display results for eight of these nine cDNAs. Sequence analyses identified five cDNAs with decreased mRNA levels in infected roots as corresponding to two putative aldolase genes, a transcription-factor TFIIA homologue, the soybean small GTP-binding protein gene <em>sra1</em>, and the soybean auxin down-regulated gene ADR12. RNA blot analyses of other auxin down-regulated genes revealed a decrease in their mRNA abundances in <em>H. glycines</em>-infected roots as well.</p>
dc.description.comments <p>This article is published as Hermsmeier, Dieter, Mitra Mazarei, and Thomas J. Baum. "Differential display analysis of the early compatible interaction between soybean and the soybean cyst nematode." <em>Molecular plant-microbe interactions</em> 11, no. 12 (1998): 1258-1263, doi: <a href="https://doi.org/10.1094/MPMI.1998.11.12.1258">10.1094/MPMI.1998.11.12.1258</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/154/
dc.identifier.articleid 1171
dc.identifier.contextkey 10539013
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/154
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57597
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/154/1998_Baum_DifferentialDisplay.pdf|||Fri Jan 14 20:40:28 UTC 2022
dc.source.uri 10.1094/MPMI.1998.11.12.1258
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Plant Pathology
dc.subject.keywords syncytium
dc.title Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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