Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative

dc.contributor.author Colonna, William
dc.contributor.author Marti, Mustafa
dc.contributor.author Nyman, John
dc.contributor.author Green, Chris
dc.contributor.author Glatz, Charles
dc.contributor.department Center for Crops Utilization Research
dc.contributor.department Chemical and Biological Engineering
dc.date 2018-02-19T05:54:41.000
dc.date.accessioned 2020-06-30T01:09:30Z
dc.date.available 2020-06-30T01:09:30Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2017-03-01
dc.description.abstract <p>If biosurfactants are to achieve their promise in environmental oil-spill remediation, their toxicity to marine life must be assessed. A killifish larvae assay is commonly used as a measure of toxicity but is difficult and nonlinear in response. Red blood cell (RBC) hemolysis has also been the basis for assays of some surfactant levels. Here we present a modified sheep RBC suspension assay and compare its response to that of the fish assay for surfactin and its genetically modified variant fatty-acyl-glutamate (FA-Glu). This is the first report of hemolytic activity as a property of FA-Glu. The method's potential for screening for toxicity against marine organisms is demonstrated.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Colonna, William J., Mustafa E. Martı, John A. Nyman, Chris Green, and Charles E. Glatz. "Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative." Environmental Progress & Sustainable Energy 36, no. 2 (2017): 505-510, which has been published in final form at <a href="http://dx.doi.org/10.1002/ep.12444" target="_blank">10.1002/ep.12444</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/306/
dc.identifier.articleid 1306
dc.identifier.contextkey 11206966
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/306
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13403
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/306/2016_Glatz_HemolysisRapid.pdf|||Fri Jan 14 23:29:08 UTC 2022
dc.source.uri 10.1002/ep.12444
dc.subject.disciplines Biological Engineering
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.disciplines Chemical Engineering
dc.title Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication ef0c8753-d288-4a4e-815f-fb800c343de8
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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