CYP701A8: A Rice ent-Kaurene Oxidase Paralog Diverted to More Specialized Diterpenoid Metabolism

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dc.contributor.author Wang, Qiang
dc.contributor.author Hillwig, Matthew
dc.contributor.author Peters, Reuben
dc.contributor.author Wu, Yisheng
dc.contributor.author Peters, Reuben
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2020-04-02T21:14:12.000
dc.date.accessioned 2020-06-29T23:46:58Z
dc.date.available 2020-06-29T23:46:58Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.issued 2012-03-01
dc.description.abstract <p>All higher plants contain an <em>ent</em>-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice (<em>Oryza sativa</em>) contains five CYP701A subfamily members in its genome, despite the fact that only one (OsKO2/CYP701A6) is required for GA biosynthesis. Here we demonstrate that one of the other rice CYP701A subfamily members, OsKOL4/CYP701A8, does not catalyze the prototypical conversion of the <em>ent</em>-kaurene C4α-methyl to a carboxylic acid, but instead carries out hydroxylation at the nearby C3α position in a number of related diterpenes. In particular, under conditions where OsKO2 catalyzes the expected conversion of <em>ent</em>-kaurene to <em>ent</em>-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with <em>ent</em>-sandaracopimaradiene and <em>ent</em>-cassadiene to produce the corresponding C3α-hydroxylated diterpenoids. These compounds are expected intermediates in biosynthesis of the oryzalexin and phytocassane families of rice antifungal phytoalexins, respectively, and can be detected in rice plants under the appropriate conditions. Thus, it appears that OsKOL4 plays a role in the more specialized diterpenoid metabolism of rice, and our results provide evidence for divergence of a KO/CYP701 family member from GA biosynthesis. This further expands the range of enzymes recruited from the ancestral GA primary pathway to the more complex and specialized labdane-related diterpenoid metabolic network found in rice.</p>
dc.description.comments <p>This article is published as Wang, Qiang, Matthew L. Hillwig, Yisheng Wu, and Reuben J. Peters. "CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism." <em>Plant physiology</em> 158, no. 3 (2012): 1418-1425. doi: <a href="https://doi.org/10.1104/pp.111.187518" target="_blank">10.1104/pp.111.187518</a>. Copyright American Society of Plant Biologists. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/276/
dc.identifier.articleid 1283
dc.identifier.contextkey 17184932
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/276
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10749
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/276/2012_Peters_RiceOxidase.pdf|||Fri Jan 14 23:07:40 UTC 2022
dc.source.uri 10.1104/pp.111.187518
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Biology
dc.title CYP701A8: A Rice ent-Kaurene Oxidase Paralog Diverted to More Specialized Diterpenoid Metabolism
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 498a24ec-81d7-4bee-b145-323d38e7a392
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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