Autophagy in the heart is enhanced and independent of disease progression in mus musculus dystrophinopathy models

dc.contributor.author Spaulding, H. R.
dc.contributor.author Selsby, Joshua
dc.contributor.author Ballmann, C.
dc.contributor.author Quindry, J. C.
dc.contributor.author Hudson, M. B.
dc.contributor.author Selsby, Joshua
dc.contributor.department Animal Science
dc.date 2021-01-28T22:01:22.000
dc.date.accessioned 2021-02-24T21:13:43Z
dc.date.available 2021-02-24T21:13:43Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-01-01
dc.description.abstract <p>Background: Duchenne muscular dystrophy is a muscle wasting disease caused by dystrophin gene mutations resulting in dysfunctional dystrophin protein. Autophagy, a proteolytic process, is impaired in dystrophic skeletal muscle though little is known about the effect of dystrophin deficiency on autophagy in cardiac muscle. We hypothesized that with disease progression autophagy would become increasingly dysfunctional based upon indirect autophagic markers.</p> <p>Methods: Markers of autophagy were measured by western blot in 7-week-old and 17-month-old control (C57) and dystrophic (mdx) hearts.</p> <p>Results: Counter to our hypothesis, markers of autophagy were similar between groups. Given these surprising results, two independent experiments were conducted using 14-month-old mdx mice or 10-month-old mdx/Utrn± mice, a more severe model of Duchenne muscular dystrophy. Data from these animals suggest increased autophagosome degradation.</p> <p>Conclusion: Together these data suggest that autophagy is not impaired in the dystrophic myocardium as it is in dystrophic skeletal muscle and that disease progression and related injury is independent of autophagic dysfunction.</p>
dc.description.comments <p>This article is published as Spaulding, H. R., C. Ballmann, J. C. Quindry, M. B. Hudson, and J. T. Selsby. "Autophagy in the heart is enhanced and independent of disease progression in mus musculus dystrophinopathy models." <em>JRSM cardiovascular disease</em> 8 (2019): 1-9. doi: <a href="https://doi.org/10.1177%2F2048004019879581">10.1177/2048004019879581</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/698/
dc.identifier.articleid 1690
dc.identifier.contextkey 21303615
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/698
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93453
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/698/2019_Selsby_AutophagyHeart.pdf|||Sat Jan 15 01:31:28 UTC 2022
dc.source.uri 10.1177/2048004019879581
dc.subject.disciplines Animal Experimentation and Research
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Cardiology
dc.subject.disciplines Physiology
dc.subject.keywords mdx
dc.subject.keywords utrophin
dc.subject.keywords heart
dc.subject.keywords Duchenne muscular dystrophy
dc.title Autophagy in the heart is enhanced and independent of disease progression in mus musculus dystrophinopathy models
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6f883eb1-dd01-4874-8949-fa2eaef5899a
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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