Common and specific genomic sequences of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization

dc.contributor.author Kariyawasam, Subhashinie
dc.contributor.author Nolan, Lisa
dc.contributor.author Scaccianoce, Jennifer
dc.contributor.author Nolan, Lisa
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:38:32.000
dc.date.accessioned 2020-07-07T05:15:03Z
dc.date.available 2020-07-07T05:15:03Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.embargo 2013-05-06
dc.date.issued 2007-08-30
dc.description.abstract <p><strong>Background</strong>: Suppression subtractive hybridization (SSH) strategy was used with extraintestinal pathogenic Escherichia coli (EXPEC) that cause avian colibacillosis (avian pathogenic E. coli or APEC) and human urinary tract infections (uropathogenic E. coli or UPEC) to determine if they possessed genes that were host and/or niche specific. Both APEC and UPEC isolates were used as tester and driver strains in 4 different SSHs in order to obtain APEC- and UPEC-specific subtraction fragments (SFs).</p> <p><strong>Results</strong>: These procedures yielded a total of 136 tester-specific SFs of which 85 were APEC-derived and 51 were UPEC-derived. Most of the APEC-derived SFs were associated with plasmids; whereas, the majority of UPEC-derived sequences matched to the bacterial chromosome. We further determined the distribution of these tester-derived sequences in a collection of UPEC and APEC isolates using polymerase chain reaction techniques. Plasmid-borne, APEC-derived sequences (tsh, cvaB, traR, traC and sopB) were predominantly present in APEC, as compared to UPEC. Of the UPEC-derived SFs, those encoding hemolysin D and F1C major and minor fimbrial subunits were present only in UPEC. However, two UPEC-derived SFs that showed strong similarity to the uropathgenic-specific protein gene (usp) occurred in APEC, demonstrating that usp is not specific to UPEC.</p> <p><strong>Conclusion</strong>: This study provides evidence of the genetic variability of ExPEC as well as genomic similarities between UPEC and APEC; it did not identify any single marker that would dictate host and/or niche specificity in APEC or UPEC. However, further studies on the genes that encode putative or hypothetical proteins might offer important insight into the pathogenesis of disease, as caused by these two ExPEC.</p>
dc.description.comments <p>This article is from <em>BMC Microbiology</em> 7 (2007): 81, doi:<a href="http://dx.doi.org/10.1186/1471-2180-7-81" target="_blank">10.1186/1471-2180-7-81</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/22/
dc.identifier.articleid 1023
dc.identifier.contextkey 4110309
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/22
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92325
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/22/2007_KariyawasamS_CommonSpecificGenomic.pdf|||Fri Jan 14 22:39:54 UTC 2022
dc.source.uri 10.1186/1471-2180-7-81
dc.subject.disciplines Genomics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Common and specific genomic sequences of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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