Association of INOS, TRAIL, TGF-b2, TGF-b3, and IgL Genes with Response to Salmonella enteritidis in Poultry

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dc.contributor.author Malek, Massoud
dc.contributor.author Lamont, Susan
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2018-02-17T06:07:24.000
dc.date.accessioned 2020-06-29T23:39:00Z
dc.date.available 2020-06-29T23:39:00Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2003
dc.date.issued 2003-01-01
dc.description.abstract <p>Several candidate genes were selected, based on their critical roles in the host’s response to intracellular bacteria, to study the genetic control of the chicken response to Salmonella enteritidis (SE). The candidate genes were: inducible nitric oxide synthase (INOS), tumor necrosis factor related apoptosis inducing ligand (TRAIL), transforming growth factor b2 (TGF-b2), transforming growth factor b3 (TGF-b3), and immunoglobulin G light chain (IgL). Responses to pathogenic SE colonization or to SE vaccination were measured in the Iowa Salmonella response resource population (ISRRP). Outbred broiler sires and three diverse, highly inbred dam lines produced 508 F1 progeny, which were evaluated as young chicks for either bacterial load isolated from spleen or cecum contents after pathogenic SE inoculation, or the circulating antibody level after SE vaccination. Fragments of each gene were sequenced from the founder lines of the resource population to identify genomic sequence variation. Single nucleotide polymorphisms (SNP) were identified, then PCR-RFLP techniques were developed to genotype the F1 resource population. Linear mixedmodels were used for statistical analyses. Because the inbred dam lines always contributed one copy of the same allele, the heterozygous sire allele effects could be assessed in the F1 generation. Association analyses revealed significant effects of the sire allele of TRAIL-StyI on the spleen (P < 0:07) and cecum (P < 0:0002) SE bacterial load. Significant effects (P < 0:04) were found on the cecum bacterial load for TGF-b3-BsrI. Varied and moderate association was found for SE vaccine antibody response for all genes. This is the first reported study on the association of SNP in INOS, TRAIL, TGF-b2, TGF-b3, and IgL with the chicken response to SE. Identification of candidate genes to improve the immune response may be useful for marker-assisted selection to enhance disease resistance.</p>
dc.description.comments <p>This article is from <em>Genetics Selection Evolution</em> 35 (2003): S99, doi:<a href="http://dx.doi.org/10.1051/gse:2003019" target="_blank">10.1051/gse:2003019</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/205/
dc.identifier.articleid 1205
dc.identifier.contextkey 7847966
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/205
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9611
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/205/2003_Lamont_AssociationINOS.pdf|||Fri Jan 14 22:25:28 UTC 2022
dc.source.uri 10.1051/gse:2003019
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords Salmonella
dc.subject.keywords genetic
dc.subject.keywords candidate gene
dc.subject.keywords PCR-RFLP
dc.subject.keywords poultry
dc.title Association of INOS, TRAIL, TGF-b2, TGF-b3, and IgL Genes with Response to Salmonella enteritidis in Poultry
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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