Function of UCHL1 Gene in Neurons using TALEN-mediated Mutagenesis

dc.contributor.author Horton, Mary
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-14T09:56:19.000
dc.date.accessioned 2020-07-07T05:10:21Z
dc.date.available 2020-07-07T05:10:21Z
dc.date.issued 2014-04-15
dc.description.abstract <p>Amyotrophic lateral sclerosis (or Lou Gehrig's disease) is a neurodegenerative disease caused by the death of motor neurons in the spinal cord and the brain. Once these nerve cells die, the patient's muscle cells degenerate, resulting in paralysis and eventually death. Most cases of ALS are sporadic, meaning that a clear molecular and genetic understanding of the mechanisms by which the motor neurons die is lacking. Ubiquitin C-terminal hydrolase-L1 (UCHL1) is one gene that has been implicated in ALS, as well as several other neurodegenerative diseases. To gain a better understanding into the function of UCHL1 in non-diseased neurons, we are using TAL-effector nucleases (TALEN) to create UCHL1 mutant zebrafish. Specifically, I have generated these TALENs and begun to inject them to generate the mutant fish. Once created, these fish will provide insights into the normal function of UCHL1 and, hopefully, allow us to create a new zebrafish model of nerve cell degeneration.</p>
dc.identifier archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/13/
dc.identifier.articleid 1012
dc.identifier.contextkey 5914571
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath undergradresearch_symposium/2014/presentations/13
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91574
dc.source.bitstream archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/13/HortonMary.pdf|||Fri Jan 14 19:36:11 UTC 2022
dc.subject.disciplines Genetics
dc.title Function of UCHL1 Gene in Neurons using TALEN-mediated Mutagenesis
dc.type event
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
thesis.degree.discipline Biology
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