Detection of Flaviviruses and Orthobunyaviruses in Mosquitoes in the Yucatan Peninsula of Mexico in 2008

Date
2010-10-22
Authors
Farfan-Ale, Jose
Loroño-Pino, Maria
Garcia-Rejon, Julian
Soto, Victor
Lin, Ming
Staley, Molly
Dorman, Karin
Bartholomay, Lyric
Hovav, Einat
Blitvich, Bradley
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Entomology
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Abstract

A total of 191,244 mosquitoes from 24 species were collected in the Yucatan Peninsula of Mexico from January to December 2008, and tested for the presence of cytopathic virus by virus isolation in Vero cells. Eighteen virus isolates were obtained, all of which were orthobunyaviruses. These were identified by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing as Cache Valley virus (n = 17) and South River virus (n = 1). A subset (n = 20,124) of Culex quinquefasciatus collected throughout the year was further tested by RT-PCR using flavivirus-specific primers. Flavivirus RNA was present in this mosquito species year-round. The overall flavivirus minimal infection rate, expressed as the number of positive mosquito pools per 1000 mosquitoes tested, was 7.7 and the monthly flavivirus minimal infection rates ranged from 4.3 to 16.6. Approximately one-third of the RT-PCR products were sequenced and all corresponded to Culex flavivirus, a recently discovered insect-specific flavivirus.

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<p>This is a copy of an article published in the <em>Vector-Borne and Zoonotic Diseases</em> ©2010 Mary Ann Liebert, Inc.; <em>Vector-Borne and Zoonotic Diseases</em> is available online at: http://online.liebertpub.com.</p>
Keywords
Veterinary Microbiology and Preventive Medicine, Genetics Development and Cell Biology
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