Comparison of Commercial Real-Time Reverse Transcription-PCR Assays for Reliable, Early, and Rapid Detection of Heterologous Strains of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected or Noninfected Boars by Use of Different Sample Types

dc.contributor.author Gerber, Priscilla
dc.contributor.author O'Neill, Kevin
dc.contributor.author Owolodun, Olajide
dc.contributor.author Wang, Chong
dc.contributor.author Wang, Chong
dc.contributor.author Harmon, Karen
dc.contributor.author Zhang, Jianqiang
dc.contributor.author Halbur, Patrick
dc.contributor.author Zhou, Lei
dc.contributor.author Meng, Xiang-Jin
dc.contributor.author Opriessnig, Tanja
dc.contributor.department Statistics
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-02-17T17:41:56.000
dc.date.accessioned 2020-07-07T05:13:26Z
dc.date.available 2020-07-07T05:13:26Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.issued 2013-01-01
dc.description.abstract <p>The aims of this study were to compare three commercial porcine reproductive and respiratory syndrome virus (PRRSV) real-time reverse transcription-PCR (RT-PCR) assays for detection of genetically diverse PRRSV isolates in serum, semen, blood swabs, and oral fluids collected from experimentally infected boars and to evaluate the effects of sample pooling. Six groups of three boars negative for PRRSV were each inoculated with one of six PRRSV isolates (sharing 55 to 99% nucleotide sequence identity in ORF5). Samples were collected on days −2, 1, 3, 5, 7, 14, and 21 postinoculation (p.i.) and tested by one of three commercially available real-time RT-PCR assays (VetMax from Applied Biosystems, Foster City, CA [abbreviated AB]; VetAlert from Tetracore, Rockville, MD [TC]; and AcuPig from AnDiaTec GmbH, Kornwestheim, Germany [AD]). At day 1 p.i., all assays detected at least one positive sample in each group. The highest detection rates were on days 3 and 5 p.i. Between days 1 and 7 p.i., serum samples had the highest detection rate (90%) with 100% agreement between tests, followed by blood swabs (kappa value of 0.97) and semen (kappa value of 0.80). Oral fluids had the lowest detection rates (AB, 55%; TC, 41%; AD, 46%) and the highest disagreement between kits (kappa value of 0.63). Pools of five samples did not reduce the detection rates if there was one positive sample with a large amount (cycle threshold, <30) of viral RNA in the pool. Serum and blood swab samples had shorter turnaround times for RNA extraction. The AB assay had a 1.6-times-shorter PCR time. In summary, serum and blood swabs had the best performance with highest detection rates and agreement between assays and the shortest turnaround times.</p>
dc.description.comments <p>This article is from <em>Journal of Clinical Microbiology </em>51 (2013); 547, doi: <a href="http://dx.doi.org/10.1128/JCM.02685-12" target="_blank">10.1128/JCM.02685-12</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/49/
dc.identifier.articleid 1061
dc.identifier.contextkey 8688997
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/49
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92076
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/49/2013_Wang_ComparisonCommercial.pdf|||Sat Jan 15 00:29:28 UTC 2022
dc.source.uri 10.1128/JCM.02685-12
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Other Veterinary Medicine
dc.subject.disciplines Statistical Methodology
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.title Comparison of Commercial Real-Time Reverse Transcription-PCR Assays for Reliable, Early, and Rapid Detection of Heterologous Strains of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected or Noninfected Boars by Use of Different Sample Types
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication b715071c-c3bd-419c-b021-0ac4702f346a
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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