The effects of ILeVO and VOTiVO on root penetration and behavior of the soybean cyst nematode, Heterodera glycines

dc.contributor.author Pandey, Santosh
dc.contributor.author Beeman, Augustine
dc.contributor.author Njus, Zach
dc.contributor.author Pandey, Santosh
dc.contributor.author Tylka, Gregory
dc.contributor.author Tylka, Gregory
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Electrical and Computer Engineering
dc.date 2020-01-23T14:12:49.000
dc.date.accessioned 2020-06-30T06:23:15Z
dc.date.available 2020-06-30T06:23:15Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.embargo 2020-03-01
dc.date.issued 2019-03-01
dc.description.abstract <p>The objective of this study was to determine the effects of ILeVO (fluopyram) and VOTiVO (<em>Bacillus firmus</em> I-1582) seed treatments on <em>Heterodera glycines</em> second-stage juvenile (J2) root penetration and behavior. In a growth chamber experiment, roots of soybeans grown from treated or untreated seeds were inoculated with <em>H. glycines</em> J2s at soil depths of 2.5, 5, or 7.5 cm. ILeVO significantly reduced <em>H. glycines</em> root penetration compared with the untreated control, but only when J2s were inoculated at a soil depth of 2.5 cm, which was near the seed. Changes in nematode behavior were assessed by collecting 60-s videos of J2s after 2 h of exposure to exudates from treated seeds or radicles from treated seeds or from soil leachates in which treated seeds were planted. X- and y-coordinates of each of the 13 reference points were recorded every hour for 24 h. A custom program analyzed and transformed the coordinates into nematode motion parameters (speed and total change in curvature). ILeVO, but not VOTiVO, seed exudates significantly reduced J2 speed relative to the untreated control. Soil leachates from ILeVO or VOTiVO treatments had no consistent effect on <em>H. glycines</em> speed or total change in curvature compared with the untreated control. In another experiment, treated or untreated seeds were incubated in wells of 6-well tissue culture plates containing 11.5% Pluronic gel. Seeds were removed after 2 h, and approximately 50 J2s then were pipetted into each well. The plates were scanned every 60 min for 24 h, and the number of J2s in each well that moved a minimum distance of ≥300 µm was determined using another custom software program. ILeVO, but not VOTiVO, significantly reduced the movement of J2 populations relative to control wells in which no seeds were added. And wells that had seeds, treated or not, yielded significantly less J2 movement compared with the no-seed control. The results of these experiments indicate that ILeVO reduces activity on <em>H. glycines</em> J2s but may not affect nematodes beyond a limited area surrounding the treated seed.</p>
dc.description.comments <p>This article is published as Beeman, Augustine Q., Zach L. Njus, Santosh Pandey, and Gregory L. Tylka. "The effects of ILeVO and VOTiVO on root penetration and behavior of the soybean cyst nematode, Heterodera glycines." <em>Plant disease</em> 103, no. 3 (2019): 392-397. doi: <a href="https://doi.org/10.1094/PDIS-02-18-0222-RE">10.1094/PDIS-02-18-0222-RE</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/251/
dc.identifier.articleid 1252
dc.identifier.contextkey 12688395
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/251
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57703
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/251/0-APS_Policy_on_Open_Access.pdf|||Fri Jan 14 22:57:26 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/251/2018_Tylka_EffectsRootManuscript.pdf|||Tue Aug 28 16:15:42 UTC 2018
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/251/2019_Tylka_EffectsRoot.pdf|||Fri Jan 14 22:57:27 UTC 2022
dc.source.uri 10.1094/PDIS-02-18-0222-RE
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Electrical and Computer Engineering
dc.subject.disciplines Plant Pathology
dc.title The effects of ILeVO and VOTiVO on root penetration and behavior of the soybean cyst nematode, Heterodera glycines
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication efdd010a-b457-4f5c-a0c8-fcef573e0e88
relation.isAuthorOfPublication 1113743f-89dc-4805-8212-529b30642102
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
relation.isOrgUnitOfPublication a75a044c-d11e-44cd-af4f-dab1d83339ff
File
Original bundle
Now showing 1 - 3 of 3
Name:
2019_Tylka_EffectsRoot.pdf
Size:
788.07 KB
Format:
Adobe Portable Document Format
Description:
Name:
0-APS_Policy_on_Open_Access.pdf
Size:
216.6 KB
Format:
Adobe Portable Document Format
Description:
Name:
2018_Tylka_EffectsRootManuscript.pdf
Size:
984.68 KB
Format:
Adobe Portable Document Format
Description:
Collections