Rheological, In Situ Printability and Cell Viability Analysis of Hydrogels for Muscle Tissue Regeneration

dc.contributor.author Lau, Sharon
dc.contributor.author Ramesh, Srikanthan
dc.contributor.author Gerdes, Sam
dc.contributor.author Lau, Sharon
dc.contributor.author Mostafavi, Azadeh
dc.contributor.author Kong, Zhenyu (James)
dc.contributor.author Johnson, Blake
dc.contributor.author Tamayol, Ali
dc.contributor.author Rao, Prahalada
dc.contributor.author Rivero, Iris
dc.contributor.department Industrial and Manufacturing Systems Engineering
dc.date 2022-01-14T20:34:22.000
dc.date.accessioned 2022-01-19T03:38:51Z
dc.date.available 2022-01-19T03:38:51Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2018
dc.date.embargo 2018-11-15
dc.date.issued 2018-01-01
dc.description.abstract <p>Advancements in additive manufacturing have made it possible to fabricate biologically relevant architectures from a wide variety of materials. Hydrogels have garnered increased attention for the fabrication of muscle tissue engineering constructs due to their resemblance to living tissue and ability to function as cell carriers. However, there is a lack of systematic approaches to screen bioinks based on their inherent properties, such as rheology, printability and cell viability. Furthermore, this study takes the critical first-step for connecting in-process sensor data with construct quality by studying the influence of printing parameters. Alginate-chitosan hydrogels were synthesized and subjected to a systematic rheological analysis. <em>In situ</em> print layer photography was utilized to identify the optimum printing parameters and also characterize the fabricated three-dimensional structures. Additionally, the scaffolds were seeded with C2C12 mouse myoblasts to test the suitability of the scaffolds for muscle tissue engineering. The results from the rheological analysis and print layer photography led to the development of a set of optimum processing conditions that produced a quality deposit while the cell viability tests indicated the suitability of the hydrogel for muscle tissue engineering applications.</p>
dc.description.comments <p>This proceeding was published as Ramesh, Srikanthan; Sam Gerdes, Sharon Lau, Azadeh Mostafavi, Zhenyu (James) Kong, Blake N. Johnson, Ali Tamayol, Prahalada Rao, and Iris V. Rivero. "Rheological, In Situ Printability and Cell Viability Analysis of Hydrogels for Muscle Tissue Regeneration." <em>Proceedings of the 29th Annual International Solid Freeform Fabrication Symposium: An Additive Manufacturing Conference</em>. Austin, TX. August 13-15, 2018, pp. 835-846. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/imse_conf/150/
dc.identifier.articleid 1175
dc.identifier.contextkey 13318089
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath imse_conf/150
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/qzXBPqLv
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/imse_conf/150/0-2018_LauSharon_PermGrant_RheologicalInSitu.pdf|||Fri Jan 14 20:34:20 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/imse_conf/150/2018_LauSharon_RheologicalInSitu.pdf|||Fri Jan 14 20:34:22 UTC 2022
dc.subject.disciplines Biology and Biomimetic Materials
dc.subject.disciplines Industrial Engineering
dc.subject.disciplines Industrial Technology
dc.subject.disciplines Polymer and Organic Materials
dc.subject.keywords Printability
dc.subject.keywords muscle regeneration
dc.subject.keywords in-situ monitoring
dc.subject.keywords bioprinting
dc.subject.keywords C2C12 myoblasts
dc.title Rheological, In Situ Printability and Cell Viability Analysis of Hydrogels for Muscle Tissue Regeneration
dc.type article
dc.type.genre presentation
dspace.entity.type Publication
relation.isAuthorOfPublication f8069293-6e01-4ff3-a53a-3f33d3fb7123