Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)

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2011_Dobbs_SelectionFree.pdf (400.18 KB)
Date
2011-01-01
Authors
Sander, Jeffry
 Dahlborg, Elizabeth
 Goodwin, Matthew
 Cade, Lindsay
 Zhang, Feng
 Cifuentes, Daniel
 Curtin, Shaun
 Blackburn, Jessica
 Thibodeau-Beganny, Stacey
 Qi, Yiping
 Pierick, Christopher
 Hoffman, Ellen
 Maeder, Morgan
 Khayter, Cyd
 Reyon, Deepak
 Dobbs, Drena
 Langenau, David
 Stupar, Robert
 Giraldez, Antonio
 Voytas, Daniel
 Peterson, Randall
 Yeh, Jing-Ruey
 Joung, J. Keith
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Altmetrics
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Abstract

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.
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This is a manuscript of an article from Nature Methods 8 (2011): 67, doi: 10.1038/nmeth.1542. Posted with permission.
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https://dr.lib.iastate.edu/handle/20.500.12876/37776
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10.1038/nmeth.1542
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