Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)

Date
2011-01-01
Authors
Sander, Jeffry
Dahlborg, Elizabeth
Goodwin, Matthew
Cade, Lindsay
Zhang, Feng
Cifuentes, Daniel
Curtin, Shaun
Blackburn, Jessica
Thibodeau-Beganny, Stacey
Qi, Yiping
Pierick, Christopher
Hoffman, Ellen
Maeder, Morgan
Khayter, Cyd
Reyon, Deepak
Dobbs, Drena
Langenau, David
Stupar, Robert
Giraldez, Antonio
Voytas, Daniel
Peterson, Randall
Yeh, Jing-Ruey
Joung, J. Keith
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Abstract

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.

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This is a manuscript of an article from Nature Methods 8 (2011): 67, doi: 10.1038/nmeth.1542. Posted with permission.

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