Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
Date
2011-01-01
Authors
Sander, Jeffry
Dobbs, Drena
Dahlborg, Elizabeth
Goodwin, Matthew
Cade, Lindsay
Zhang, Feng
Cifuentes, Daniel
Curtin, Shaun
Blackburn, Jessica
Thibodeau-Beganny, Stacey
Qi, Yiping
Pierick, Christopher
Hoffman, Ellen
Maeder, Morgan
Khayter, Cyd
Reyon, Deepak
Dobbs, Drena
Langenau, David
Stupar, Robert
Giraldez, Antonio
Voytas, Daniel
Peterson, Randall
Yeh, Jing-Ruey
Joung, J. Keith
Dobbs, Drena
Dahlborg, Elizabeth
Goodwin, Matthew
Cade, Lindsay
Zhang, Feng
Cifuentes, Daniel
Curtin, Shaun
Blackburn, Jessica
Thibodeau-Beganny, Stacey
Qi, Yiping
Pierick, Christopher
Hoffman, Ellen
Maeder, Morgan
Khayter, Cyd
Reyon, Deepak
Dobbs, Drena
Langenau, David
Stupar, Robert
Giraldez, Antonio
Voytas, Daniel
Peterson, Randall
Yeh, Jing-Ruey
Joung, J. Keith
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Dobbs, Drena
Person
Research Projects
Organizational Units
Genetics, Development and Cell Biology
Organizational Unit
Bioinformatics and Computational Biology
Organizational Unit
Journal Issue
Series
Department
Genetics, Development and Cell BiologyBioinformatics and Computational Biology
Abstract
Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.
Comments
This is a manuscript of an article from Nature Methods 8 (2011): 67, doi: 10.1038/nmeth.1542. Posted with permission.