Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA) Sander, Jeffry Dobbs, Drena Dahlborg, Elizabeth Goodwin, Matthew Cade, Lindsay Zhang, Feng Cifuentes, Daniel Curtin, Shaun Blackburn, Jessica Thibodeau-Beganny, Stacey Qi, Yiping Pierick, Christopher Hoffman, Ellen Maeder, Morgan Khayter, Cyd Reyon, Deepak Dobbs, Drena Langenau, David Stupar, Robert Giraldez, Antonio Voytas, Daniel Peterson, Randall Yeh, Jing-Ruey Joung, J. Keith
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Bioinformatics and Computational Biology 2018-02-18T05:06:49.000 2020-06-30T04:01:07Z 2020-06-30T04:01:07Z Sat Jan 01 00:00:00 UTC 2011 2011-01-01
dc.description.abstract <p>Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.</p>
dc.description.comments <p>This is a manuscript of an article from <em>Nature Methods </em>8 (2011): 67, doi: <a href="" target="_blank">10.1038/nmeth.1542</a>. Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1114
dc.identifier.contextkey 9763837
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/112
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 18:44:50 UTC 2022
dc.source.uri 10.1038/nmeth.1542
dc.subject.disciplines Bioinformatics
dc.subject.disciplines Computational Biology
dc.subject.disciplines Genetics
dc.subject.disciplines Genomics
dc.subject.disciplines Zoology
dc.title Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
dc.type article
dc.type.genre article
dspace.entity.type Publication
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