Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)

dc.contributor.author Sander, Jeffry
dc.contributor.author Dobbs, Drena
dc.contributor.author Dahlborg, Elizabeth
dc.contributor.author Goodwin, Matthew
dc.contributor.author Cade, Lindsay
dc.contributor.author Zhang, Feng
dc.contributor.author Cifuentes, Daniel
dc.contributor.author Curtin, Shaun
dc.contributor.author Blackburn, Jessica
dc.contributor.author Thibodeau-Beganny, Stacey
dc.contributor.author Qi, Yiping
dc.contributor.author Pierick, Christopher
dc.contributor.author Hoffman, Ellen
dc.contributor.author Maeder, Morgan
dc.contributor.author Khayter, Cyd
dc.contributor.author Reyon, Deepak
dc.contributor.author Dobbs, Drena
dc.contributor.author Langenau, David
dc.contributor.author Stupar, Robert
dc.contributor.author Giraldez, Antonio
dc.contributor.author Voytas, Daniel
dc.contributor.author Peterson, Randall
dc.contributor.author Yeh, Jing-Ruey
dc.contributor.author Joung, J. Keith
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Bioinformatics and Computational Biology
dc.date 2018-02-18T05:06:49.000
dc.date.accessioned 2020-06-30T04:01:07Z
dc.date.available 2020-06-30T04:01:07Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2011
dc.date.issued 2011-01-01
dc.description.abstract <p>Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.</p>
dc.description.comments <p>This is a manuscript of an article from <em>Nature Methods </em>8 (2011): 67, doi: <a href="http://dx.doi.org/10.1038/nmeth.1542" target="_blank">10.1038/nmeth.1542</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/112/
dc.identifier.articleid 1114
dc.identifier.contextkey 9763837
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/112
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37776
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/112/2011_Dobbs_SelectionFree.pdf|||Fri Jan 14 18:44:50 UTC 2022
dc.source.uri 10.1038/nmeth.1542
dc.subject.disciplines Bioinformatics
dc.subject.disciplines Computational Biology
dc.subject.disciplines Genetics
dc.subject.disciplines Genomics
dc.subject.disciplines Zoology
dc.title Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 7e096c4f-9007-41e4-9414-989c3ea9bc88
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
relation.isOrgUnitOfPublication c331f825-0643-499a-9eeb-592c7b43b1f5
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