Characterization of a Novel Porcine Parvovirus Tentatively Designated PPV5

dc.contributor.author Xiao, Chao-Ting
dc.contributor.author Giménez-Lirola, Luis
dc.contributor.author Jiang, Yong-Hou
dc.contributor.author Halbur, Patrick
dc.contributor.author Opriessnig, Tanja
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date.accessioned 2022-05-26T23:54:50Z
dc.date.available 2022-05-26T23:54:50Z
dc.date.issued 2013-06-07
dc.description.abstract A new porcine parvovirus (PPV), provisionally designated as PPV5, was identified in U.S. pigs. Cloning and sequencing from a circular or head-to-tail concatemeric array revealed that the PPV5 possesses the typical genomic organization of parvoviruses with two major predicted open reading frames (ORF1 and ORF2), and is most closely related to PPV4 with overall genomic identities of 64.1–67.3%. The amino acid identities between PPV5 and PPV4 were 84.6%–85.1% for ORF1 and 54.0%–54.3% for ORF2. Unlike PPV4, but similar to bovine parvovirus 2 (BPV2), PPV5 lacks the additional ORF3 and has a much longer ORF2. Moreover, the amino acid sequences of ORF1 and ORF2 of BPV2 showed higher homologies to PPV5 than to PPV4. The conserved motifs of the Ca2+ binding loop (YXGXG) and the catalytic center (HDXXY) of phospholipase A2 (PLA2) were identified in VP1 (ORF2) of PPV5, as well as in BPV2, but were not present in PPV4. Phylogenetic analyses revealed that PPV5, PPV4 and BPV2 form a separate clade different from the genera Parvovirus and Bocavirus. Further epidemiologic investigations of PPV4 and PPV5 in U.S. pigs of different ages indicated a slightly higher prevalence for PPV5 (6.6%; 32/483) compared to PPV4 (4.1%; 20/483), with detection of concurrent PPV4 and PPV5 in 15.6% (7/45) of lungs of infected pigs. Evidence for potential vertical transmission or association with reproductive failure was minimal for both PPV4 and PPV5. The high similarity to PPV4 and the lack of ORF3 may suggest PPV5 is an intermediate of PPV4 during the evolution of parvoviruses in pigs.
dc.description.comments This article is published as Xiao, Chao-Ting, Luis G. Gimenez-Lirola, Yong-Hou Jiang, Patrick G. Halbur, and Tanja Opriessnig. "Characterization of a novel porcine parvovirus tentatively designated PPV5." PLoS ONE 8, no. 6 (2013): e65312. DOI: 10.1371/journal.pone.0065312. Copyright 2013 Xiao et al. Attribution 4.0 International (CC BY 4.0). Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/qzoDXpPw
dc.language.iso en
dc.publisher PLoS
dc.source.uri https://doi.org/10.1371/journal.pone.0065312 *
dc.title Characterization of a Novel Porcine Parvovirus Tentatively Designated PPV5
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication abbf44fd-a4a0-4859-8b5c-af2b853c9547
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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