IRE1, a component of the unfolded protein response signaling pathway, protects pollen development in Arabidopsis from heat stress Deng, Yan Srivastava, Renu Quilichini, Teagen Dong, Haili Bao, Yan Horner, Harry Horner, Harry Howell, Stephen
dc.contributor.department Computer Science
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Plant Sciences Institute 2020-02-06T21:31:21.000 2020-06-30T04:02:08Z 2020-06-30T04:02:08Z Fri Jan 01 00:00:00 UTC 2016 2016-10-01
dc.description.abstract <p>The unfolded protein response (UPR) is activated by various stresses during vegetative development in Arabidopsis, but is constitutively active in anthers of unstressed plants. To understand the role of the UPR during reproductive development, we analyzed a double mutant, ire1a ire1b. The double mutant knocks out the RNA splicing arm of the UPR signaling pathway and is fertile at room temperature, but is male sterile at modestly elevated temperature (ET). The conditional male sterility in the mutant is a sporophytic trait, and when the double mutant was grown at ET, defects appeared in the structure of the tapetum. As a result, the tapetum in the double mutant failed to properly deposit the pollen coat at ET, which made pollen grains clump and prevented their normal dispersal. IRE1 is a dual protein kinase/ribonuclease involved in the splicing of bZIP60 mRNA, and through complementation analysis of various mutant forms of IRE1b, it was demonstrated that the ribonuclease activity of IRE1 was required for protecting male fertility from ET. It was also found that overexpression of SEC31A rescued the conditional male sterility in the double mutant. SEC31A is involved in ER to Golgi trafficking and a major target of the IRE1-mediated UPR signaling in stressed seedlings. Thus, IRE1, a major component of the UPR, plays an important role in protecting pollen development from ET.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Deng, Yan, Renu Srivastava, Teagen D. Quilichini, Haili Dong, Yan Bao, Harry T. Horner, and Stephen H. Howell. "IRE 1, a component of the unfolded protein response signaling pathway, protects pollen development in Arabidopsis from heat stress." <em>The Plant Journal</em> 88, no. 2 (2016): 193-204, which has been published in final form at doi: <a href="">10.1111/tpj.13239</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.</p>
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dc.identifier archive/
dc.identifier.articleid 1239
dc.identifier.contextkey 16474150
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/235
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 22:48:53 UTC 2022
dc.source.uri 10.1111/tpj.13239
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Numerical Analysis and Scientific Computing
dc.subject.disciplines Plant Pathology
dc.subject.keywords endoplasmic reticulum (ER)
dc.subject.keywords heat stress
dc.subject.keywords male gametophyte
dc.subject.keywords tapetum
dc.subject.keywords vesicle trafficking
dc.subject.keywords Sec31
dc.title IRE1, a component of the unfolded protein response signaling pathway, protects pollen development in Arabidopsis from heat stress
dc.type article
dc.type.genre article
dspace.entity.type Publication
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