Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves

dc.contributor.author Brito-Goulart, Débora
dc.contributor.author Wu, Zuowei
dc.contributor.author Adiguzel, Mehmet Cemal
dc.contributor.author Schroeder, Anastasia
dc.contributor.author Singh, Kritika
dc.contributor.author Xu, Changyun
dc.contributor.author Ocal, Melda Meral
dc.contributor.author Dewell, Renee
dc.contributor.author Dewell, Grant
dc.contributor.author Plummer, Paul
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Center for Food Security and Public Health
dc.date.accessioned 2022-04-23T00:16:44Z
dc.date.available 2022-04-23T00:16:44Z
dc.date.issued 2022-04-15
dc.description.abstract Campylobacter is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) Campylobacter in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in C. jejuni, 30 commercial calves were divided into Group 1, Group 2, and Group 3 (n = 10), and were all inoculated orally with FQ-susceptible (FQ-S) C. jejuni; seven days later, Group 3 was challenged with transtracheal Mannheimia haemolytica, and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R Campylobacter via culture. Before oral inoculation with C. jejuni, 87% of calves were naturally colonized by FQ-R C. jejuni. Two days after the inoculation, FQ-R C. jejuni decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all C. jejuni populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R C. jejuni populations following the injection was due mainly to enrichment of preexisting FQ-R C. jejuni, rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant Campylobacter in cattle in response to FQ treatment.
dc.description.comments This article is published as Goulart, Debora Brito, Ashenafi Feyisa Beyi, Zuowei Wu, Mehmet Cemal Adiguzel, Anastasia Schroeder, Kritika Singh, Changyun Xu et al. "Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves." Antibiotics 11, no. 4 (2022): 531. DOI: 10.3390/antibiotics11040531. Copyright 2022 The Authors. Attribution 4.0 International (CC BY 4.0). Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/kv7k61pv
dc.language.iso en
dc.publisher MDPI
dc.source.uri https://doi.org/10.3390/antibiotics11040531 *
dc.subject.keywords Campylobacter
dc.subject.keywords cattle
dc.subject.keywords colonization
dc.subject.keywords rectal feces
dc.subject.keywords bovine respiratory disease (BRD)
dc.subject.keywords fluoroquinolone/danofloxacin treatment
dc.subject.keywords antimicrobial resistance
dc.subject.keywords pulsed-field gel electrophoresis
dc.subject.keywords minimum inhibitory concentration
dc.title Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves
dc.type Article
dspace.entity.type Publication
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