High-Resolution Radiation Hybrid Mapping of Human Chromosome 17 Genes to the Pig Genome Extends Gene Order Conservation with Pig Chromosome 12 and Limited Synteny with Pig Chromosome 2

Abstract

<p>A comparative study of human chromosome 17 (HSA17) and pig chromosome 12 (SSC12) and 2 (SSC2) was conducted using both somatic cell hybrid panel (SCHP) and radiation hybrid (RH) panel analysis. A cDNA library from an expressed sequence tag (EST) project in pig reproduction was examined and six genes and ESTs from HSA17 were selected for this study. The genes/ESTs were TATA box binding protein-associated factor (<em>TAF2N/RBP56</em>), plasmin inhibitor (<em>PLI</em>), H3 histone family 3B (<em>H3F3B</em>), aminopeptidase puromycin sensitive (<em>NPEPPS/PSA</em>), P311 protein (<em>P311</em>), and an expressed sequence tag (<em>ESTMI015</em>). SCHP analysis mapped five genes/ESTs (<em>TAF2N</em> , <em>H3F3B</em>, <em>PLI</em>, <em>NPEPPS</em>, and <em>ESTMI015</em>) to SSC12q11-q15 and SSC12p11-p15 with 100% concordance, and assigned <em>P311</em> to SSC2 (1/2q24)-q29 with 100% concordance. RH analysis of all six genes confirmed the SCHP mapping results, with average retention frequency of 25%. An expanded comparative SSC12 RH map integrating the five new type I markers and 23 previously mapped loci was established using a LOD score threshold of 4.8. The gene order of the five genes/ESTs on the SSC12 framework RH map (<em>H3F3B</em>-<em>ESTMI015</em>-<em>PSA</em>-<em>RBP56</em>-<em>PLI</em>) is identical to the HSA17 GB4 map but with map inversion. Although synteny conservation between HSA17 and SSC2 has not been detected by previous painting experiments, the mapping of <em>P311</em> to SSC2 suggests that some synteny conservation exists between HSA17 and SSC2.</p>