Influence of carbon to nitrogen ratios on soybean somatic embryo (cv. Jack) growth and composition

dc.contributor.author Truong, Quyen
dc.contributor.author Shanks, Jacqueline
dc.contributor.author Koch, Kaelynn
dc.contributor.author Yoon, Jong
dc.contributor.author Everard, John
dc.contributor.author Shanks, Jacqueline
dc.contributor.department Chemical and Biological Engineering
dc.date 2018-02-15T23:55:39.000
dc.date.accessioned 2020-06-30T01:08:46Z
dc.date.available 2020-06-30T01:08:46Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.embargo 2015-03-18
dc.date.issued 2013-06-01
dc.description.abstract <p>Soybean [Glycine max (L.) Merr.] seed are valued for their protein and oil content. Soybean somatic embryos cultured in Soybean Histodifferentiation and Maturation (SHaM) medium were examined for their suitability as a model system for developing an understanding of assimilate partitioning and metabolic control points for protein and oil biosynthesis in soybean seed. This report describes the growth dynamics and compositional changes of SHaM embryos in response to change in the carbon to nitrogen ratio of the medium. It was postulated that at media compositions that were sufficient to support maximal growth rates, changes in the C:N ratio are likely to influence the partitioning of resources between the various storage products, especially protein and oil. As postulated, at steady-state growth rates, embryo protein content was strongly correlated with decreasing C:N ratios and increasing glutamine consumption rates. However, oil content remained relatively unchanged across the C:N ratio range tested, and resources were instead directed towards the starch and residual biomass (estimated by mass balance) pools in response to increasing C:N ratios. Protein and oil were inversely related only at concentrations of sucrose in the medium <88mM, where carbon limited growth and no starch was found to accumulate in the tissues. These observations and the high reproducibility in the data indicate that SHaM embryos are an ideal model system for the application of metabolic flux analysis studies designed to test hypotheses regarding assimilate partitioning in developing soybean seeds.</p>
dc.description.comments <p>This is an article from <em>Journal of Experimental Botany</em> 64 (2013): 2985, doi: <a href="http://dxldoi.org/10.1093/jxb/ert138" target="_blank">10.1093/jxb/ert138</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/252/
dc.identifier.articleid 1251
dc.identifier.contextkey 6857701
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/252
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13343
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/252/2013_ShanksJV_InfluenceCarbonNitrogen.pdf|||Fri Jan 14 22:57:42 UTC 2022
dc.source.uri 10.1093/jxb/ert138
dc.subject.disciplines Biological Engineering
dc.subject.disciplines Chemical Engineering
dc.subject.keywords Cell growth
dc.subject.keywords glutamine
dc.subject.keywords glycine max
dc.subject.keywords oil
dc.subject.keywords protein
dc.subject.keywords soluble sugars
dc.subject.keywords somatic embryogenesis
dc.subject.keywords starch
dc.subject.keywords sucrose
dc.title Influence of carbon to nitrogen ratios on soybean somatic embryo (cv. Jack) growth and composition
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 7d38b16f-fd4e-4ffc-b3bc-804e8fd2ddef
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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