Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory

dc.contributor.author Jin, Tao
dc.contributor.author Käldström, Mats
dc.contributor.author Benavides, Adriana
dc.contributor.author Kaufman Rechulski, Marcelo
dc.contributor.author Jarboe, Laura
dc.contributor.department Chemical and Biological Engineering
dc.date 2020-02-07T15:58:14.000
dc.date.accessioned 2020-06-30T01:10:19Z
dc.date.available 2020-06-30T01:10:19Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.issued 2020-02-03
dc.description.abstract <p>Mechanocatalysis is a promising method for depolymerization of lignocellulosic biomass. Microbial utilization of the resulting oligosaccharides is one potential route of adding value to the depolymerized biomass. However, it is unclear how readily these oligosaccharides are utilized by standard cell factories. Here, we investigate utilization of cellulose subjected to mechanocatalytic depolymerization, using ethanologenic <em>Escherichia coli</em> as a model fermentation organism. The mechanocatalytic oligosaccharides supported ethanol titers similar to those observed when glucose was provided at comparable concentrations. Tracking of the various oligomers, using maltose (alpha-1,4) and cellobiose (beta-1,4) oligomers as representative standards of the orientation, but not linkage, of the glycosidic bond, suggests that the malto-like-oligomers are more readily utilized than cello-like-oligomers, consistent with poor growth with cellotetraose or cellopentaose as sole carbon source. Thus, mechanocatalytic oligosaccharides are a promising substrate for cell factories, and microbial utilization of these sugars could possibly be improved by addressing utilization of cello-like oligomers.</p>
dc.description.comments <p>This article is published as Jin, Tao, Mats Käldström, Adriana Benavides, Marcelo D. Kaufman Rechulski, and Laura R. Jarboe. "Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory." <em>AMB Express</em> 10 (2020): 28. DOI: <a href="http://dx.doi.org/10.1186/s13568-020-0965-4" target="_blank">10.1186/s13568-020-0965-4</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/407/
dc.identifier.articleid 1408
dc.identifier.contextkey 16480518
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/407
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13511
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/407/2020_JarboeLaura_UtilizationMechanocatalytic.pdf|||Sat Jan 15 00:08:45 UTC 2022
dc.source.uri 10.1186/s13568-020-0965-4
dc.subject.disciplines Catalysis and Reaction Engineering
dc.subject.disciplines Microbiology
dc.subject.disciplines Oil, Gas, and Energy
dc.subject.keywords Biofuels
dc.subject.keywords Fermentation
dc.subject.keywords Coli
dc.subject.keywords Mechanocatalytic
dc.subject.keywords Oligosaccharides
dc.title Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6113d68a-37ba-4092-8ed5-44d66391fae4
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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