Nucleation of Iron Oxide Nanoparticles Mediated by Mms6 Protein in Situ

dc.contributor.author Kashyap, Sanjay
dc.contributor.author Woehl, Taylor
dc.contributor.author Liu, Xunpei
dc.contributor.author Mallapragada, Surya
dc.contributor.author Prozorov, Tanya
dc.contributor.department Chemical and Biological Engineering
dc.date 2018-02-16T22:28:25.000
dc.date.accessioned 2020-06-30T01:08:51Z
dc.date.available 2020-06-30T01:08:51Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-01-01
dc.description.abstract <p>Biomineralization proteins are widely used as templating agents in biomimetic synthesis of a variety of organic-inorganic nanostructures. However, the role of the protein in controlling the nucleation and growth of biomimetic particles is not well understood, because the mechanism of the bioinspired reaction is often deduced from ex situ analysis of the resultant nanoscale mineral phase. Here we report the direct visualization of biomimetic iron oxide nanoparticle nucleation mediated by an acidic bacterial recombinant protein, Mms6, during an in situ reaction induced by the controlled addition of sodium hydroxide to solution-phase Mms6 protein micelles incubated with ferric chloride. Using in situ liquid cell scanning transmission electron microscopy we observe the liquid iron prenucleation phase and nascent amorphous nanoparticles forming preferentially on the surface of protein micelles. Our results provide insight into the early steps of protein-mediated biomimetic nucleation of iron oxide and point to the importance of an extended protein surface during nanoparticle formation.</p>
dc.description.comments <p>Reprinted (adapted) with permission from <em>ACS Nano</em> 8 (2014): 9097, doi: <a href="http://dx.doi.org/10.1021/nn502551y" target="_blank">10.1021/nn502551y</a>. Copyright 2014 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/261/
dc.identifier.articleid 1263
dc.identifier.contextkey 7480147
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/261
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13353
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/261/0-2014_MallapragadaSK_NucleationIronOxide.pdf|||Fri Jan 14 23:02:07 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/261/2014_MallapragadaSK_NucleationIronOxide.pdf|||Fri Jan 14 23:02:08 UTC 2022
dc.source.uri 10.1021/nn502551y
dc.subject.disciplines Biological Engineering
dc.subject.disciplines Chemical Engineering
dc.subject.keywords recombinant iron-binding protein
dc.subject.keywords protein-mediated particle nucleation
dc.subject.keywords in situ TEM analysis
dc.title Nucleation of Iron Oxide Nanoparticles Mediated by Mms6 Protein in Situ
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication cf742fe7-cf8d-4bf5-98bd-00b8a45db967
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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