Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance

Date
2017-09-01
Authors
Jin, Tao
Rover, Marjorie R.
Petersen, Elspeth
Chi, Zhanyou
Smith, Ryan G.
Brown, Robert C.
Wen, Zhiyou
Jarboe, Laura R.
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press
Altmetrics
Authors
Research Projects
Organizational Units
Journal Issue
Series
Department
Chemical and Biological EngineeringBioinformatics and Computational BiologyMicrobiologyFood Science and Human Nutrition
Abstract
Lignocellulosic biomass is an appealing feedstock for the production of biorenewable fuels and chemicals, and thermochemical processing is a promising method for depolymerizing it into sugars. However, trace compounds in this pyrolytic sugar syrup are inhibitory to microbial biocatalysts. This study demonstrates that hydrophobic inhibitors damage the cell membrane of ethanologenic Escherichia coli KO11+lgk. Adaptive evolution was employed to identify design strategies for improving pyrolytic sugar tolerance and utilization. Characterization of the resulting evolved strain indicates that increased resistance to the membrane-damaging effects of the pyrolytic sugars can be attributed to a glutamine to leucine mutation at position 29 of carbon storage regulator CsrA. This single amino acid change is sufficient for decreasing EPS protein production and increasing membrane integrity when exposed to pyrolytic sugars.
Comments
This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Industrial Microbiology and Biotechnology following peer review. The version of record: Jin, Tao, Marjorie R. Rover, Elspeth M. Petersen, Zhanyou Chi, Ryan G. Smith, Robert C. Brown, Zhiyou Wen, and Laura R. Jarboe. "Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance." Journal of Industrial Microbiology and Biotechnology 44, no. 9 (2017): 1279-1292 is available online at DOI: 10.1007/s10295-017-1958-4. Copyright 2017 Society for Industrial Microbiology and Biotechnology. Posted with permission.
Description
Keywords
DegreeDisciplines::Engineering::Chemical Engineering::Biochemical and Biomolecular Engineering, DegreeDisciplines::Physical Sciences and Mathematics::Environmental Sciences::Oil, Gas, and Energy
Citation
DOI
Collections