Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance

dc.contributor.author Jin, Tao
dc.contributor.author Rover, Marjorie R.
dc.contributor.author Petersen, Elspeth
dc.contributor.author Chi, Zhanyou
dc.contributor.author Smith, Ryan G.
dc.contributor.author Brown, Robert C.
dc.contributor.author Wen, Zhiyou
dc.contributor.author Jarboe, Laura R.
dc.contributor.department Chemical and Biological Engineering
dc.contributor.department Bioinformatics and Computational Biology
dc.contributor.department Microbiology
dc.contributor.department Food Science and Human Nutrition
dc.date.accessioned 2022-02-15T21:57:08Z
dc.date.available 2022-02-15T21:57:08Z
dc.date.issued 2017-09-01
dc.description.abstract Lignocellulosic biomass is an appealing feedstock for the production of biorenewable fuels and chemicals, and thermochemical processing is a promising method for depolymerizing it into sugars. However, trace compounds in this pyrolytic sugar syrup are inhibitory to microbial biocatalysts. This study demonstrates that hydrophobic inhibitors damage the cell membrane of ethanologenic Escherichia coli KO11+lgk. Adaptive evolution was employed to identify design strategies for improving pyrolytic sugar tolerance and utilization. Characterization of the resulting evolved strain indicates that increased resistance to the membrane-damaging effects of the pyrolytic sugars can be attributed to a glutamine to leucine mutation at position 29 of carbon storage regulator CsrA. This single amino acid change is sufficient for decreasing EPS protein production and increasing membrane integrity when exposed to pyrolytic sugars.
dc.description.comments This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Industrial Microbiology and Biotechnology following peer review. The version of record: Jin, Tao, Marjorie R. Rover, Elspeth M. Petersen, Zhanyou Chi, Ryan G. Smith, Robert C. Brown, Zhiyou Wen, and Laura R. Jarboe. "Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance." Journal of Industrial Microbiology and Biotechnology 44, no. 9 (2017): 1279-1292 is available online at DOI: 10.1007/s10295-017-1958-4. Copyright 2017 Society for Industrial Microbiology and Biotechnology. Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/WwPgeqRz
dc.language.iso en
dc.publisher Oxford University Press
dc.source.uri https://doi.org/10.1007/s10295-017-1958-4 *
dc.subject DegreeDisciplines::Engineering::Chemical Engineering::Biochemical and Biomolecular Engineering
dc.subject DegreeDisciplines::Physical Sciences and Mathematics::Environmental Sciences::Oil, Gas, and Energy
dc.title Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication 2608f992-004b-43c0-a1bc-597c6b50b946
relation.isAuthorOfPublication 06f45e8f-14d0-42f9-90df-c902e3049987
relation.isAuthorOfPublication 6113d68a-37ba-4092-8ed5-44d66391fae4
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
relation.isOrgUnitOfPublication c331f825-0643-499a-9eeb-592c7b43b1f5
relation.isOrgUnitOfPublication 76818945-1d8c-461a-bbac-df615c052c9e
relation.isOrgUnitOfPublication 4b6428c6-1fda-4a40-b375-456d49d2fb80
Original bundle
Now showing 1 - 1 of 1
331.57 KB
Adobe Portable Document Format