Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions in Cuticular Lipid Deposition

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2020-07-01
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Alexander, Liza Esther
Okazaki, Yozo
Schelling, Michael
Davis, Aeriel
Zheng, Xiaobin
Rizhsky, Ludmila
Saito, Kazuki
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Yandeau-Nelson, Marna
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Nikolau, Basil
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Genetics, Development and Cell Biology

The Department of Genetics, Development, and Cell Biology seeks to teach subcellular and cellular processes, genome dynamics, cell structure and function, and molecular mechanisms of development, in so doing offering a Major in Biology and a Major in Genetics.

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The Department of Genetics, Development, and Cell Biology was founded in 2005.

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Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
Abstract

Plant epidermal cells express unique molecular machinery that juxtapose the assembly of intracellular lipid components and the unique extracellular cuticular lipids that are unidirectionally secreted to plant surfaces. In maize (Zea mays), mutations at the glossy2 (gl2) locus affect the deposition of extracellular cuticular lipids. Sequence-based genome scanning identified a new Gl2 homolog in the maize genome, namely Gl2-like. Both the Gl2-like and Gl2 genes are members of the BAHD superfamily of acyltransferases, with close sequence similarity to the Arabidopsis (Arabidopsis thaliana) CER2 gene. Transgenic experiments demonstrated that Gl2-like and Gl2 functionally complement the Arabidopsis cer2 mutation, with differential influences on the cuticular lipids and the lipidome of the plant, particularly affecting the longer alkyl chain acyl lipids, especially at the 32-carbon chain length. Site-directed mutagenesis of the putative BAHD catalytic HXXXDX-motif indicated that Gl2-like requires this catalytic capability to fully complement the cer2 function, but Gl2 can accomplish complementation without the need for this catalytic motif. These findings demonstrate that Gl2 and Gl2-like overlap in their cuticular lipid function, but have evolutionarily diverged to acquire nonoverlapping functions.

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This article is published as Alexander, Liza Esther, Yozo Okazaki, Michael A. Schelling, Aeriel Davis, Xiaobin Zheng, Ludmila Rizhsky, Marna D. Yandeau-Nelson, Kazuki Saito, and Basil J. Nikolau. "Maize Glossy2 and Glossy2-like genes have overlapping and distinct functions in cuticular lipid deposition." Plant Physiology 183, no. 3 (2020): 840-853. doi:10.1104/pp.20.00241.

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Wed Jan 01 00:00:00 UTC 2020
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