Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions in Cuticular Lipid Deposition

dc.contributor.author Alexander, Liza Esther
dc.contributor.author Okazaki, Yozo
dc.contributor.author Schelling, Michael
dc.contributor.author Nikolau, Basil
dc.contributor.author Davis, Aeriel
dc.contributor.author Zheng, Xiaobin
dc.contributor.author Rizhsky, Ludmila
dc.contributor.author Yandeau-Nelson, Marna
dc.contributor.author Saito, Kazuki
dc.contributor.author Nikolau, Basil
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Center for Metabolic Biology
dc.date 2021-08-26T21:07:58.000
dc.date.accessioned 2021-09-09T16:16:41Z
dc.date.available 2021-09-09T16:16:41Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.issued 2020-07-01
dc.description.abstract <p>Plant epidermal cells express unique molecular machinery that juxtapose the assembly of intracellular lipid components and the unique extracellular cuticular lipids that are unidirectionally secreted to plant surfaces. In maize (<em>Zea mays</em>), mutations at the <em>glossy2 (gl2)</em> locus affect the deposition of extracellular cuticular lipids. Sequence-based genome scanning identified a new <em>Gl2</em> homolog in the maize genome, namely <em>Gl2-like</em>. Both the <em>Gl2-like</em> and <em>Gl2</em> genes are members of the BAHD superfamily of acyltransferases, with close sequence similarity to the Arabidopsis (<em>Arabidopsis thaliana</em>) <em>CER2</em> gene. Transgenic experiments demonstrated that <em>Gl2-like</em> and <em>Gl2</em> functionally complement the Arabidopsis <em>cer2</em> mutation, with differential influences on the cuticular lipids and the lipidome of the plant, particularly affecting the longer alkyl chain acyl lipids, especially at the 32-carbon chain length. Site-directed mutagenesis of the putative BAHD catalytic HXXXDX-motif indicated that <em>Gl2-like</em> requires this catalytic capability to fully complement the <em>cer2</em> function, but <em>Gl2</em> can accomplish complementation without the need for this catalytic motif. These findings demonstrate that <em>Gl2</em> and <em>Gl2-like</em> overlap in their cuticular lipid function, but have evolutionarily diverged to acquire nonoverlapping functions.</p>
dc.description.comments <p>This article is published as Alexander, Liza Esther, Yozo Okazaki, Michael A. Schelling, Aeriel Davis, Xiaobin Zheng, Ludmila Rizhsky, Marna D. Yandeau-Nelson, Kazuki Saito, and Basil J. Nikolau. "Maize Glossy2 and Glossy2-like genes have overlapping and distinct functions in cuticular lipid deposition." <em>Plant Physiology</em> 183, no. 3 (2020): 840-853. doi:<a href="https://doi.org/10.1104/pp.20.00241">10.1104/pp.20.00241</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/316/
dc.identifier.articleid 1326
dc.identifier.contextkey 24513625
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/316
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/EzR2Ee8z
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/316/0-CC_license_for_2020_Nikolau_MaizeGlossy.pdf|||Fri Jan 14 23:32:25 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/316/2020_Nikolau_MaizeGlossy.pdf|||Fri Jan 14 23:32:26 UTC 2022
dc.source.uri 10.1104/pp.20.00241
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Sciences
dc.title Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions in Cuticular Lipid Deposition
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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