Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions in Cuticular Lipid Deposition Alexander, Liza Esther Okazaki, Yozo Schelling, Michael Nikolau, Basil Davis, Aeriel Zheng, Xiaobin Rizhsky, Ludmila Yandeau-Nelson, Marna Saito, Kazuki Nikolau, Basil
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Center for Metabolic Biology 2021-08-26T21:07:58.000 2021-09-09T16:16:41Z 2021-09-09T16:16:41Z Wed Jan 01 00:00:00 UTC 2020 2020-07-01
dc.description.abstract <p>Plant epidermal cells express unique molecular machinery that juxtapose the assembly of intracellular lipid components and the unique extracellular cuticular lipids that are unidirectionally secreted to plant surfaces. In maize (<em>Zea mays</em>), mutations at the <em>glossy2 (gl2)</em> locus affect the deposition of extracellular cuticular lipids. Sequence-based genome scanning identified a new <em>Gl2</em> homolog in the maize genome, namely <em>Gl2-like</em>. Both the <em>Gl2-like</em> and <em>Gl2</em> genes are members of the BAHD superfamily of acyltransferases, with close sequence similarity to the Arabidopsis (<em>Arabidopsis thaliana</em>) <em>CER2</em> gene. Transgenic experiments demonstrated that <em>Gl2-like</em> and <em>Gl2</em> functionally complement the Arabidopsis <em>cer2</em> mutation, with differential influences on the cuticular lipids and the lipidome of the plant, particularly affecting the longer alkyl chain acyl lipids, especially at the 32-carbon chain length. Site-directed mutagenesis of the putative BAHD catalytic HXXXDX-motif indicated that <em>Gl2-like</em> requires this catalytic capability to fully complement the <em>cer2</em> function, but <em>Gl2</em> can accomplish complementation without the need for this catalytic motif. These findings demonstrate that <em>Gl2</em> and <em>Gl2-like</em> overlap in their cuticular lipid function, but have evolutionarily diverged to acquire nonoverlapping functions.</p>
dc.description.comments <p>This article is published as Alexander, Liza Esther, Yozo Okazaki, Michael A. Schelling, Aeriel Davis, Xiaobin Zheng, Ludmila Rizhsky, Marna D. Yandeau-Nelson, Kazuki Saito, and Basil J. Nikolau. "Maize Glossy2 and Glossy2-like genes have overlapping and distinct functions in cuticular lipid deposition." <em>Plant Physiology</em> 183, no. 3 (2020): 840-853. doi:<a href="">10.1104/pp.20.00241</a>.</p>
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dc.identifier archive/
dc.identifier.articleid 1326
dc.identifier.contextkey 24513625
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/316
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 23:32:25 UTC 2022
dc.source.bitstream archive/|||Fri Jan 14 23:32:26 UTC 2022
dc.source.uri 10.1104/pp.20.00241
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Sciences
dc.title Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions in Cuticular Lipid Deposition
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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