Raman Imaging in Cell Membranes, Lipid-Rich Organelles, and Lipid Bilayers

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dc.contributor.author Syed, Aleem
dc.contributor.author Smith, Emily
dc.contributor.author Smith, Emily
dc.contributor.department Ames Laboratory
dc.contributor.department Chemistry
dc.date 2020-04-10T01:18:50.000
dc.date.accessioned 2020-06-30T01:17:36Z
dc.date.available 2020-06-30T01:17:36Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.issued 2017-06-01
dc.description.abstract <p>Raman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous Raman scattering suffers from a low intensity that hinders its use in some cellular applications. However, developments in coherent Raman imaging, surface-enhanced Raman imaging, and tip-enhanced Raman imaging have enabled video-rate imaging, excellent detection limits, and nanometer spatial resolution, respectively. After a brief introduction to these commonly used Raman imaging techniques for cell membrane studies, this review discusses selected applications of these modalities for chemical imaging of membrane proteins and lipids. Finally, recent developments in chemical tags for Raman imaging and their applications in the analysis of selected cell membrane components are summarized. Ongoing developments toward improving the temporal and spatial resolution of Raman imaging and small-molecule tags with strong Raman scattering cross sections continue to expand the utility of Raman imaging for diverse cell membrane studies.</p>
dc.description.comments <p>This is a manuscript of an article published as Syed, Aleem, and Emily A. Smith. "Raman imaging in cell membranes, lipid-rich organelles, and lipid bilayers." <em>Annual Review of Analytical Chemistry</em> 10 (2017): 271-291. DOI: <a href="https://doi.org/10.1146/annurev-anchem-061516-045317" target="_blank">10.1146/annurev-anchem-061516-045317</a>. Posted with permission from the <em>Annual Review of Analytical Chemistry,</em> http://www.annualreviews.org.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/chem_pubs/1226/
dc.identifier.articleid 2234
dc.identifier.contextkey 17327076
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath chem_pubs/1226
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/14538
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/1226/2017_SmithEmily_RamanImaging.pdf|||Fri Jan 14 19:16:48 UTC 2022
dc.source.uri 10.1146/annurev-anchem-061516-045317
dc.subject.disciplines Analytical Chemistry
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Cancer Biology
dc.subject.disciplines Medicinal-Pharmaceutical Chemistry
dc.subject.keywords coherent anti-Stokes Raman scattering
dc.subject.keywords stimulated Raman scattering
dc.subject.keywords surface-enhanced Raman scattering
dc.subject.keywords tip-enhanced Raman scattering
dc.subject.keywords alkyne tags
dc.subject.keywords deuterium labeling
dc.title Raman Imaging in Cell Membranes, Lipid-Rich Organelles, and Lipid Bilayers
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1593ba6f-2eb3-4290-ab73-86b7601506fa
relation.isOrgUnitOfPublication 25913818-6714-4be5-89a6-f70c8facdf7e
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
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