Biochemical characterization of new P450s in rice diterpenoid metabolism

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dc.contributor.author Brown, Benjamin
dc.contributor.department Biochemistry, Biophysics, and Molecular Biology
dc.date 2018-02-17T18:48:52.000
dc.date.accessioned 2020-06-30T04:33:48Z
dc.date.available 2020-06-30T04:33:48Z
dc.date.issued 2016-04-01
dc.description.abstract <p>The Oryza sativa P450 CYP76M subfamily hydroxylate a range of labdane-related diterpenes (LRD). Previously the CYP76M5-8 enzymes were screened against the range of LRDs found in rice using our metabolic engineering system in Escherichia coli (E. coli) to determine their functions. It was found that, even though these P450s all have very similar amino acid sequences, individual enzymes in this family have different specificities e.g., CYP76M8 is a more promiscuous enzyme, hydroxylating a wider range of LRDs than CYP76M7. This study focuses on screening two more recently discovered P450s in this same family, CYP76M14 & 17. CYP76M14 was found to have a high specificity for a few LRD, whereas CYP76M17 was more promiscuous. This poster will present the data comparing the functions of these six enzymes.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/honors_posters/201604/projects/4/
dc.identifier.articleid 1037
dc.identifier.contextkey 8864112
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath honors_posters/201604/projects/4
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/42414
dc.relation.ispartofseries Honors Projects and Posters
dc.source.bitstream archive/lib.dr.iastate.edu/honors_posters/201604/projects/4/Brown__Benjamin.pdf|||Sat Jan 15 00:01:06 UTC 2022
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.title Biochemical characterization of new P450s in rice diterpenoid metabolism
dc.type event
dc.type.genre article
dspace.entity.type Publication
relation.isSeriesOfPublication 78a1cb49-0dee-4c38-97a8-c1fd0b7a74ea
thesis.degree.discipline Agricultural Biochemistry
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