G-Wires: Self-Assembly of a Telomeric Oligonucleotide, d(GGGGTTGGGG), into Large Superstructures

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1994-09-01
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Marsh, Thomas
Henderson, Eric
Henderson, Eric
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Zoology and Genetics
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The telomeric DNA oligonucleotide 5’-G4T~G4-3’ (Tet 1.5) spontaneously assembles into large superstructures we have termed G-wires. G-wires can be resolved by gel electrophoresis as a ladder pattern. The self-association of Tet 1.5 is noncovalent and exhibits characteristics of G4-DNA, a parallel fourstranded structure stabilized by guanine tetrads. Formation of G-wires is dependent upon the presence of Na+ and/or K+, and once formed, G-wires are resistant to denaturation. The results described here extend our understanding of the structural potential of G-rich nucleic acids and may provide insight into the possible roles of G-rich sequences and the novel structures they can form in biological systems.

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<p>Reprinted (adapted) with permission from G-Wires: Self-Assembly of a Telomeric Oligonucleotide, d(GGGGTTGGGG), into Large Superstructures. Thomas C. Marsh and Eric Henderson Biochemistry <strong>1994</strong> <em>33</em> (35), 10718-10724. DOI: 10.1021/bi00201a020. Copyright 1994 American Chemical Society.</p>
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