Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids

dc.contributor.author Magtoto, Ronaldo
dc.contributor.author Armenta-Leyva, Betsy
dc.contributor.author Dizon-Magtoto, Precy
dc.contributor.author Cheng, Ting-Yu
dc.contributor.author Clavijo, Maria J.
dc.contributor.author Johnson, Clayton
dc.contributor.author Lopez, Will
dc.contributor.author Baum, David
dc.contributor.author Zimmerman, Jeffrey
dc.contributor.author Giménez-Lirola, Luis
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date.accessioned 2022-05-09T18:21:58Z
dc.date.available 2022-05-09T18:21:58Z
dc.date.issued 2022-05
dc.description.abstract The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.
dc.description.comments This article is published as Magtoto, Ronaldo, Betsy Armenta-Leyva, Precy Dizon-Magtoto, Ting-Yu Cheng, Maria J. Clavijo, Clayton Johnson, Will Lopez, David Baum, Jeffrey Zimmerman, and Luis G. Giménez-Lirola. "Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids." Journal of Swine Health and Production 30, no. 3 (2022): 165-170. DOI: 10.54846/jshap/1265. Copyright 2022 AASV. Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/dvmq3O4v
dc.language.iso en
dc.publisher American Association of Swine Veterinarians
dc.source.uri https://doi.org/10.54846/jshap/1265 *
dc.subject.keywords swine
dc.subject.keywords Mycoplasma hyopneumoniae
dc.subject.keywords processing fluid
dc.subject.keywords enzyme-linked immunosorbent assay
dc.subject.keywords diagnostic performance
dc.title Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication abbf44fd-a4a0-4859-8b5c-af2b853c9547
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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