Quantifying Attachment and Antibiotic Resistance of Escherichia coli from Conventional and Organic Swine Manure

dc.contributor.author Jarboe, Laura
dc.contributor.author Zwonitzer, Martha
dc.contributor.author Soupir, Michelle
dc.contributor.author Jarboe, Laura
dc.contributor.author Soupir, Michelle
dc.contributor.author Smith, Douglas
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2018-02-17T14:31:08.000
dc.date.accessioned 2020-06-29T22:42:06Z
dc.date.available 2020-06-29T22:42:06Z
dc.date.issued 2016-03-01
dc.description.abstract <p>Broad-spectrum antibiotics are often administered to swine, contributing to the occurrence of antibiotic-resistant bacteria in their manure. During land application, the bacteria in swine manure preferentially attach to particles in the soil, affecting their transport in overland flow. However, a quantitative understanding of these attachment mechanisms is lacking, and their relationship to antibiotic resistance is unknown. The objective of this study is to examine the relationships between antibiotic resistance and attachment to very fine silica sand in<em>Escherichia coli</em> collected from swine manure. A total of 556 isolates were collected from six farms, two organic and four conventional (antibiotics fed prophylactically). Antibiotic resistance was quantified using 13 antibiotics at three minimum inhibitory concentrations: resistant, intermediate, and susceptible. Of the 556 isolates used in the antibiotic resistance assays, 491 were subjected to an attachment assay. Results show that <em>E. coli</em> isolates from conventional systems were significantly more resistant to amoxicillin, ampicillin, chlortetracycline, erythromycin, kanamycin, neomycin, streptomycin, tetracycline, and tylosin (<em>P</em> < 0.001). Results also indicate that <em>E. coli</em> isolated from conventional systems attached to very fine silica sand at significantly higher levels than those from organic systems (<em>P</em> < 0.001). Statistical analysis showed that a significant relationship did not exist between antibiotic resistance levels and attachment in <em>E. coli</em> from conventional systems but did for organic systems (<em>P</em> < 0.001). Better quantification of these relationships is critical to understanding the behavior of <em>E. coli</em> in the environment and preventing exposure of human populations to antibiotic-resistant bacteria.</p>
dc.description.comments <p>This article is from <em>Journal of Environmental Quality</em> 45 (2016): 609–617, doi:<a href="http://dx.doi.org/10.2134/jeq2015.05.0245" target="_blank">10.2134/jeq2015.05.0245</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/718/
dc.identifier.articleid 2005
dc.identifier.contextkey 8317423
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/718
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/1513
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/718/2016_Zwonitzer_QuantifyingAttachment.pdf|||Sat Jan 15 01:43:33 UTC 2022
dc.source.uri 10.2134/jeq2015.05.0245
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.title Quantifying Attachment and Antibiotic Resistance of Escherichia coli from Conventional and Organic Swine Manure
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6113d68a-37ba-4092-8ed5-44d66391fae4
relation.isAuthorOfPublication 04becbfb-7a97-4d96-a0dd-5514295530ee
relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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