Absence of glyphosate-induced effects on ovarian folliculogenesis and steroidogenesis

dc.contributor.author Ganesan, Shanthi
dc.contributor.author Keating, Aileen
dc.contributor.author McGuire, Bailey
dc.contributor.author Keating, Aileen
dc.contributor.department Animal Science
dc.date 2020-06-29T21:27:09.000
dc.date.accessioned 2020-06-29T23:41:39Z
dc.date.available 2020-06-29T23:41:39Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.embargo 2021-06-24
dc.date.issued 2020-06-24
dc.description.abstract <p>Glyphosate (GLY) is an herbicidal active ingredient and both in vitro and in vivo studies suggest that GLY alters ovarian function. To determine if a chronic GLY exposure model affected steroidogenesis or folliculogenesis in vivo, postnatal day 42 C57BL6 female mice were orally delivered vehicle control (saline) or GLY (2 mg/Kg) from a pipette tip five days per week for either five or ten weeks. Mice were euthanized at the pro-estrus stage of the estrous cycle. GLY exposure did not impact body weight gain, organ weights, or healthy follicle numbers. In addition, GLY exposure did not affect abundance of ovarian mRNA encoding kit ligand (Kitlg), KIT protooncogene receptor tyrosine kinase (c-Kit), insulin receptor (Insr), insulin receptor substrate (Irs1 or Irs2) and protein thymoma viral proto-oncogene 1 (AKT) or phosphorylated AKT. Ovarian mRNA or protein abundance of Star, 3β-hydroxysteroid dehydrogenase (Hsd3b1), Cyp11a1 or Cyp19a were also not altered by GLY. Circulating 17β-estradiol and progesterone concentration were unaffected by GLY exposure. In conclusion, chronic GLY exposure for five or ten weeks did not affect the ovarian endpoints examined herein.</p>
dc.description.comments <p>This is a manuscript of an article published as Ganesan, Shanthi, Bailey C. McGuire, and Aileen F. Keating. "Absence of glyphosate-induced effects on ovarian folliculogenesis and steroidogenesis." <em>Reproductive Toxicology</em> (2020). doi: <a href="https://doi.org/10.1016/j.reprotox.2020.06.011" target="_blank" title="Persistent link using digital object identifier">10.1016/j.reprotox.2020.06.011</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/542/
dc.identifier.articleid 1543
dc.identifier.contextkey 18311601
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/542
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9981
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/542/2020_Keating_AbsenceGlyphosateManuscript.pdf|||Sat Jan 15 00:53:48 UTC 2022
dc.source.uri 10.1016/j.reprotox.2020.06.011
dc.subject.disciplines Animal Experimentation and Research
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Toxicology
dc.subject.keywords Glyphosate
dc.subject.keywords PI3K pathway
dc.subject.keywords folliculogenesis
dc.subject.keywords steroidogenesis
dc.title Absence of glyphosate-induced effects on ovarian folliculogenesis and steroidogenesis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication abe31007-187a-4174-864e-6871e3f9a0d7
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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