Requirements of renewable fuels around the world increases daily as the need to reduce greenhouse gas emissions increase as well. Biofuels are one of the several alternative energy sources being heavily invested into by both world governments and corporations to combat both issues. This project focuses on testing the toxicity of common biofuels in Escherichia coli (E. coli) for better use in the fermentation process. Our goal is to improve tolerance to inhibitors produced after treatment of lignocellulose, allowing for better conversions in biofuels. This will result in an increase in production of biofuels and a drop of the land requirements, making them more economically available to the general publIC.