Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds
This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.
This chapter is from Scott, M. Paul. "Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds." Transgenic Maize: Methods and Protocols (2009): 123-128, doi: 10.1007/978-1-59745-494-0_10.