Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds
| dc.contributor.author | Scott, M. Paul | |
| dc.contributor.department | Department of Agronomy | |
| dc.date | 2018-02-18T16:05:38.000 | |
| dc.date.accessioned | 2020-06-29T23:03:10Z | |
| dc.date.available | 2020-06-29T23:03:10Z | |
| dc.date.issued | 2009-01-01 | |
| dc.description.abstract | <p>This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.</p> | |
| dc.description.comments | <p>This chapter is from Scott, M. Paul. "Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds." <em>Transgenic Maize: Methods and Protocols</em> (2009): 123-128, doi: <a href="http://dx.doi.org/10.1007/978-1-59745-494-0_10" target="_blank">10.1007/978-1-59745-494-0_10</a>.</p> | |
| dc.format.mimetype | application/pdf | |
| dc.identifier | archive/lib.dr.iastate.edu/agron_pubs/179/ | |
| dc.identifier.articleid | 1186 | |
| dc.identifier.contextkey | 10446724 | |
| dc.identifier.s3bucket | isulib-bepress-aws-west | |
| dc.identifier.submissionpath | agron_pubs/179 | |
| dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/4509 | |
| dc.language.iso | en | |
| dc.source.bitstream | archive/lib.dr.iastate.edu/agron_pubs/179/2009_Scott_TissuePrint.pdf|||Fri Jan 14 21:30:38 UTC 2022 | |
| dc.source.uri | 10.1007/978-1-59745-494-0_10 | |
| dc.subject.disciplines | Agricultural Science | |
| dc.subject.disciplines | Agronomy and Crop Sciences | |
| dc.subject.disciplines | Plant Breeding and Genetics | |
| dc.subject.keywords | Seed | |
| dc.subject.keywords | Endosperm | |
| dc.subject.keywords | Antibody | |
| dc.subject.keywords | Protein | |
| dc.title | Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds | |
| dc.type | article | |
| dc.type.genre | book_chapter | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 97acee5f-1291-4c27-8929-a8e1617c411d | |
| relation.isOrgUnitOfPublication | fdd5c06c-bdbe-469c-a38e-51e664fece7a |
File
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- 2009_Scott_TissuePrint.pdf
- Size:
- 156.66 KB
- Format:
- Adobe Portable Document Format
- Description: