Tissue expression and antibacterial activity of host defense peptides in chicken

Thumbnail Image
Date
2016-10-13
Authors
Lee, Mi Ok
Jang, Hyun-Jun
Rengaraj, Deivendran
Yang, Seo-Yeong
Han, Jae Yong
Lamont, Susan
Womack, James
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Authors
Person
Research Projects
Organizational Units
Organizational Unit
Journal Issue
Is Version Of
Versions
Series
Department
Animal Science
Abstract

Background Host defence peptides are a diverse group of small, cationic peptides and are important elements of the first line of defense against pathogens in animals. Expression and functional analysis of host defense peptides has been evaluated in chicken but there are no direct, comprehensive comparisons with all gene family and individual genes.

Results We examined the expression patterns of all known cathelicidins, β-defensins and NK-lysin in multiple selected tissues from chickens. CATH1 through 3 were predominantly expressed in the bone marrow, whereas CATHB1 was predominant in bursa of Fabricius. The tissue specific pattern of β-defensins generally fell into two groups. β-defensin1-7 expression was predominantly in bone marrow, whereas β-defensin8-10 and β-defensin13 were highly expressed in liver. NK-lysin expression was highest in spleen. We synthesized peptide products of these gene families and analysed their antibacterial efficacy. Most of the host defense peptides showed antibacterial activity against E.coli with dose-dependent efficacy. β-defensin4 and CATH3 displayed the strongest antibacterial activity among all tested chicken HDPs. Microscopic analyses revealed the killing of bacterium by disrupting membranes with peptide treatment.

Conclusions These results demonstrate dose-dependent antimicrobial effects of chicken HDPs mediated by membrane damage and demonstrate the differential tissue expression pattern of bioactive HDPs in chicken and the relative antimicrobial potency of the peptides they encode.

Comments

This article is published as Lee, Mi Ok, Hyun-Jun Jang, Deivendran Rengaraj, Seo-Yeong Yang, Jae Yong Han, Susan J. Lamont, and James E. Womack. "Tissue expression and antibacterial activity of host defense peptides in chicken." BMC Veterinary Research 12, (2016): 231. DOI: 10.1186/s12917-016-0866-6. Posted with permission.

Description
Keywords
Citation
DOI
Copyright
Fri Jan 01 00:00:00 UTC 2016
Collections