Tissue expression and antibacterial activity of host defense peptides in chicken

dc.contributor.author Lee, Mi Ok
dc.contributor.author Jang, Hyun-Jun
dc.contributor.author Lamont, Susan
dc.contributor.author Rengaraj, Deivendran
dc.contributor.author Yang, Seo-Yeong
dc.contributor.author Han, Jae Yong
dc.contributor.author Lamont, Susan
dc.contributor.author Womack, James
dc.contributor.department Animal Science
dc.date 2020-10-07T02:47:27.000
dc.date.accessioned 2021-02-24T21:11:52Z
dc.date.available 2021-02-24T21:11:52Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-10-13
dc.description.abstract <p><strong>Background</strong> Host defence peptides are a diverse group of small, cationic peptides and are important elements of the first line of defense against pathogens in animals. Expression and functional analysis of host defense peptides has been evaluated in chicken but there are no direct, comprehensive comparisons with all gene family and individual genes.</p> <p><strong>Results</strong> We examined the expression patterns of all known cathelicidins, β-defensins and NK-lysin in multiple selected tissues from chickens. CATH1 through 3 were predominantly expressed in the bone marrow, whereas CATHB1 was predominant in bursa of Fabricius. The tissue specific pattern of β-defensins generally fell into two groups. β-defensin1-7 expression was predominantly in bone marrow, whereas β-defensin8-10 and β-defensin13 were highly expressed in liver. NK-lysin expression was highest in spleen. We synthesized peptide products of these gene families and analysed their antibacterial efficacy. Most of the host defense peptides showed antibacterial activity against E.coli with dose-dependent efficacy. β-defensin4 and CATH3 displayed the strongest antibacterial activity among all tested chicken HDPs. Microscopic analyses revealed the killing of bacterium by disrupting membranes with peptide treatment.</p> <p><strong>Conclusions</strong> These results demonstrate dose-dependent antimicrobial effects of chicken HDPs mediated by membrane damage and demonstrate the differential tissue expression pattern of bioactive HDPs in chicken and the relative antimicrobial potency of the peptides they encode.</p>
dc.description.comments <p>This article is published as Lee, Mi Ok, Hyun-Jun Jang, Deivendran Rengaraj, Seo-Yeong Yang, Jae Yong Han, Susan J. Lamont, and James E. Womack. "Tissue expression and antibacterial activity of host defense peptides in chicken." <em>BMC Veterinary Research</em> 12, (2016): 231. DOI: <a href="https://doi.org/10.1186/s12917-016-0866-6" target="_blank">10.1186/s12917-016-0866-6</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/589/
dc.identifier.articleid 1584
dc.identifier.contextkey 19683150
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/589
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93344
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/589/2016_LamontSusan_TissueExpression.pdf|||Sat Jan 15 01:02:40 UTC 2022
dc.source.uri 10.1186/s12917-016-0866-6
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Poultry or Avian Science
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Host defence peptides (HDPs)
dc.subject.keywords Antimicrobial
dc.subject.keywords Chicken
dc.title Tissue expression and antibacterial activity of host defense peptides in chicken
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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