Conference Proceedings and Presentations
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PublicationDiversity in Digital Architectural Perspectives: Focuses on Human Figures(Design Communication Association, 2022-11-28)Recent articles have addressed how human figures in architectural perspectives play an essential role in evoking viewers' empathy and representing diversity, equity, and inclusion in design. Although inclusivity in architectural design is vital for successful design and communication, this study found the types of human figures placed in renderings and how the rendering style expresses diversity in age, skin color, disability, gender, etc. This study conducted a content analysis with 138 visual images collected from a professional architectural competition organizer. The images were analyzed to investigate how diverse human figures have been used in rending design outcomes.
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PublicationPredictors of Offender Treatment Attrition in the Iowa Domestic Abuse Program( 2021-05)The rate of offender treatment attrition for batterer intervention programs (BIPs) is high and of great concern as treatment noncompletion is predictive of poor treatment outcomes and domestic assault recidivism, the latter of which poses a threat to public safety. Attrition in cognitive behavioral therapy (CBT) and the Duluth Model, the traditional standards for BIPs, has been extensively studied while the research on attrition in other BIP models, such as Achieving Change Through Values-Based Behavior (ACTV), has been limited. The current study examined predictors of attrition by comparing pre-treatment differences between treatment completers and noncompleters. The sample included 3,474 men in the Iowa Domestic Abuse Program (IDAP) (Duluth/CBT or ACTV), a court mandated BIP, who were convicted of domestic assault against an intimate partner. The results indicated that demographic factors such as age, race, and education level were significant predictors of attrition, while marital status was not a significant predictor. Criminal history factors such as the total number of charges and the number of domestic assault charges before the IDAP start date were also not significant predictors of attrition. These findings provide suggestions to improve BIP treatment delivery and indicate a greater need to expand research to new BIP models.
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PublicationCreating and Testing of Cas9 Delivering DNA Origami Nanosystem( 2021-05)DNA origami is a self-assembly method in which a single stranded DNA (ssDNA) molecule called the “scaffold” via an ensemble of smaller ssDNA molecules called “staples” into a precision nanoparticle. DNA origami can be heavily modified for in vivo biocompatibility and targeting making it a promising method for delivering DNA in vivo. The goal of this project was to create a DNA origami nanoparticle comprised of Cas9 and a guide RNA (gRNA) and deliver it to Zebrafish (Danio rerio) embryos to assay for in vivo gene editing. In preliminary experiments the gene for Green Fluorescent Protein (GFP) was used as a Cas9 proxy. GFP origami was validated by gel electrophoresis and Atomic Force Microscopy (AFM). However, no fluorescence was observed after injecting with either the GFP origami or positive controls (linear GFP gene and a GFP plasmid). The failure of positive controls suggests technical errors in the procedure. Thus, further experimentation is required with appropriate controls to determine if the use of DNA origami for gene delivery has merit.
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PublicationArtificial Seed Drying Conditions and Maize Seed Viability and Vigor after Storage( 2021-05)Transposable elements (TEs) are DNA sequences that can change positions within the genome and may induce mutations. In Maize and Arabidopsis genomes, environmental stressors may cause an increase in transposition events. We used X-ray radiation on maize (W22) and arabidopsis (TAIR10) and then assembled a bioinformatic pipeline to test for an increase in TEs. Arabidopsis was exposed to 80 Gy of radiation and Maize was exposed to 10 Gy. Each plant was grown and self-crossed for three generations before sample collection was performed to ensure the fixation of activated TEs. Oxford Nanopore long-read sequencing was performed, and reads were aligned to their respective reference genome using minimap2. SVIM, a bioinformatics tool for detecting structural variants, was used to find instances of novel insertions (NI) in the genomes. The NI were then compared to the reference genome using the BLAST software, and the BLAST hits showed where the NI aligned with the reference genome. The BLAST hits were then compared to the annotated transposons via bedtools intersect and R code. Instances where the start and endpoints of the BLAST hits are close to the start and endpoints of the annotated transposons will be identified in order to locate active transposons.
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Publication"Our Community Carol" - Theatrical Evolution in a Post-COVID World( 2021-05)This project discusses and showcases how live theatre has evolved during the pandemic to still provide opportunity and accessibility to both the performers and the audience. It looks at the creation of and final product of ISU Theatre's COVID-safe production of the play "Our Community Carol" and the innovations needed to make a project like that work without losing its theatricality during that height of the pandemic. The project also looks at ISU Theatre's live streamed musical production "Songs For a New World" as well as general innovations to modern theatre to increase accessibility for all audiences, and innovations that could still be made.