Plant-in-chip: Microfluidic system for studying root growth and pathogenic interactions in Arabidopsis
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2011-06-29
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AIP Publishing LLC
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Electrical and Computer Engineering
Abstract
We report a microfluidic platform for the hydroponic growth of Arabidopsis plants with high-resolution visualization of root development and root-pathogen interactions. The platform comprises a set of parallel microchannels with individual input/output ports where 1-day old germinated seedlings are initially placed. Under optimum conditions, a root system grows in each microchannel and its images are recorded over a 198-h period. Different concentrations of plant growth media show different root growth characteristics. Later, the developed roots are inoculated with two plant pathogens (nematodes and zoospores) and their physicochemical interactions with the live root systems are observed.
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This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Parashar, Archana, and Santosh Pandey. "Plant-in-chip: Microfluidic system for studying root growth and pathogenic interactions in Arabidopsis." Applied Physics Letters 98, no. 26 (2011): 263703, and may be found at DOI: 10.1063/1.3604788. Copyright 2011 American Institute of Physics. Posted with permission.