Label free thrombin detection in presence of high concentration of albumin using an aptamer-functionalized nanoporous membrane
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2019-02-01
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Elsevier B.V.
Abstract
Nanoporous alumina membranes have become a ubiquitous biosensing platform for a variety of applications and aptamers are being increasingly utilized as recognition elements in protein sensing devices. Combining the advantages of the two, we report label-free sensitive detection of human α-thrombin by an aptamer-functionalized nanoporous alumina membrane using a four- electrode electrochemical cell. The sensor response to α-thrombin was determined in the presence of a high concentration (500 μM) of human serum albumin (HSA) as an interfering protein in the background. The sensor sensitivity was also characterized against γ-thrombin, which is a modified α-thrombin lacking the aptamer binding epitope. The detection limit, within an appreciable signal/noise ratio, was 10 pM of α-thrombin in presence of 500 μM HSA. The proposed scheme involves the use of minimum reagents/sample preparation steps, has appreciable response in presence of high concentrations of interfering molecules and is readily amenable to miniaturization by association with existing-chip based electrical systems for application in point-of-care diagnostic devices.
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This is a manuscript of an article published as Gosai, Agnivo, Brendan Shin Hau Yeah, Marit Nilsen-Hamilton, and Pranav Shrotriya. "Label free thrombin detection in presence of high concentration of albumin using an aptamer-functionalized nanoporous membrane." Biosensors and Bioelectronics 126 (2019): 88-95. doi:10.1016/j.bios.2018.10.010. © 2018 Elsevier B.V. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.