Salmonella enterica subsp. enterica serovar Typhimurium Phage Type DT104 contains a region where five major antibiotic genes are located, which has been named Salmonella Genomic Island 1 (SGI1) (Boyd, et al, 2002) and bound by Class I integrons which have been shown to aid in transferring genes horizontally. Antibiotic resistance profiles, Pulsed Field Gel Electrophoresis (PFGE) and Polymerase Chain Reaction (PCR) were used to determine if these genes are present in the serovars.