Effects of phosvitin phosphopeptide-Ca complex prepared by efficient enzymatic hydrolysis on calcium absorption and bone deposition of mice
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2022-11
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Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
Abstract
Phosvitin (PV) was treated with high-temperature, mild pressure (HTMP), and enzyme combination, and then phosvitin phosphopeptides-calcium (PPP-Ca) complexes were prepared. The low-calcium specific pathogen free-Kunming (SPF-KM) mice were used to determine the effect of PPP-Ca complexes on intestinal calcium absorption and their utilization for bone formation. The serum calcium content was the highest with the HTMP-Enz-PPP-Ca treatment (2.19 mmol/L), and it significantly down-regulated the abnormal elevation of serum alkaline phosphatase (AKP) caused by calcium deficiency. The low-calcium control group had the lowest calcium deposited to the femur (80.41 mg/g) and the lowest femur bone mineral density (BMD) (0.17 g/cm3), while HTMP-Enz-PPP-Ca significantly improved bone calcium content (94.33 mg/g) and BMD (0.29 g/cm3). The micro-computed tomography (MCT) images showed that the femur with the normal control, PV-Ca, and HTMP-Enz-PPP-Ca treatments had a more compact, complete, and thicker trabecular network than the low-calcium and CaCl2 treatments. These results indicated that the organic calcium (HTMP-Enz-PPP-Ca) promoted calcium absorption and bone deposition, and the effect of HTMP-Enz-PPP-Ca was better than the inorganic CaCl2.
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This article is published as Zhao, Mengdie, Dong Uk Ahn, Songming Li, Wei Liu, Shengwei Yi, and Xi Huang. "Effects of phosvitin phosphopeptide-Ca complex prepared by efficient enzymatic hydrolysis on calcium absorption and bone deposition of mice." Food Science and Human Wellness 11, no. 6 (2022): 1631-1640. doi:10.1016/j.fshw.2022.06.022. © 2022 Beijing Academy of Food Sciences.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).